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Method for rapidly detecting melamine

A technology of melamine and cyanuric acid, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problems of high detection cost, high false positive rate, and difficulty in popularization, etc. problem, to achieve the effect of low detection cost and easy operation

Active Publication Date: 2009-03-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Around 1990, Raman spectroscopy, near-infrared, and mid-infrared techniques have been reported abroad for the detection of melamine, but these three methods require advanced precision instruments, are highly technical, and difficult to popularize, and are not suitable for rapid detection of melamine on site.
Although it has been reported to use enzyme-linked immunosorbent assay kits for detection, the detection cost is high and the false positive rate is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Add petroleum ether to 20 mL of liquid milk at a volume ratio of 1:1, centrifuge at 5000 rpm for 10 minutes, and remove the upper layer of fat. Add 5% mercuric sulfate aqueous solution at a volume ratio of 1:1, and stir well. The liquid milk after stirring is added into activated carbon according to the weight volume ratio of 0.5% for 10 minutes, and then vacuum-filtered, and the clarified filtrate is collected. Then the filtrate was heated and concentrated 10 times and then cooled to room temperature. Add 1.5mL of 5% acetic acid dropwise into the concentrated solution at a volume ratio of 1:1, mix well, let it stand for 1min, observe that the solution is turbid, it can be determined that the melamine content in the liquid milk sample is greater than or equal to 2mg / kg.

Embodiment 2

[0026] Add ether to 20mL of liquid milk at a volume ratio of 2:1, centrifuge at 5000 rpm for 10 minutes, and remove the upper layer of fat. Add 5% zinc chloride aqueous solution at a volume ratio of 1:1 and stir well. The liquid milk after stirring is added into active carbon according to the weight volume ratio of 0.75% to absorb for 10 minutes, and then vacuum-filtered, and the clarified filtrate is collected. Then the filtrate was heated and concentrated 10 times and then cooled to room temperature. Add 1.5mL of 5% oxalic acid dropwise into the concentrated solution at a volume ratio of 3:2, add and mix evenly, let stand for 1min, observe that the solution is turbid, it can be determined that the melamine content in the liquid milk sample is greater than or equal to 2mg / kg.

Embodiment 3

[0028] Add n-hexane to 10 mL of liquid milk at a volume ratio of 2:1, centrifuge at 5000 rpm for 10 minutes, and remove the upper layer of fat. Add 5% lead acetate aqueous solution at a volume ratio of 1:1, and stir well. The liquid milk after stirring is added into active carbon according to the weight volume ratio of 1% to absorb for 10 minutes, and then vacuum-filtered, and the clarified filtrate is collected. Then the filtrate was heated and concentrated 10 times and then cooled to room temperature. Add 1.5mL of 5% tannic acid dropwise into the concentrated solution at a volume ratio of 1:1, mix well, and let it stand for 1min. If the solution is observed to be cloudy, it can be determined that the melamine content in the liquid milk sample is greater than or equal to 2mg / kg.

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Abstract

The invention discloses a detection method of melamine, belonging to the fast detection method for the adulteration components of food and feeds. The technical scheme of the detection method comprises the steps of: (1) processing impurity removal pretreatment on an object sample to obtain filtrate; (2) adding 5-10% acetic acid, 1-5% cyanuric acid, 5-10% oxalic acid or 5-10% tannic acid into the filtrate, visually observing the generation of turbidity, and if there is turbidity generation, considering the melamine content of the sample higher than 2mg / kg. The detection method is used for the melamine of milk products and feeds, which is characterized in specificity, high efficiency, high speed, accuracy and stability. The detection method has simple operation and low detection cost, thus is beneficial for actual popularization and application, to satisfy the actual demands of liquid milk, powdered milk and feed enterprises and the like and provide a fast and effective detection method for the melamine of food and feed under government supervision.

Description

technical field [0001] The invention relates to a method for detecting melamine, which belongs to a method for rapidly detecting adulterated ingredients in food and feed. Background technique [0002] Melamine (Melamine) referred to as triamine, scientific name triamine triazine, alias melamine, cyanuramide, tripolyamide, molecular formula is C 3 N 6 h 6 , with a relative molecular weight of 126.12, is a kind of nitrogen heterocyclic compound. Melamine is soluble in methanol, formaldehyde, acetic acid, hot glycol, glycerin, pyridine; slightly soluble in water, ethanol; insoluble in ether, benzene and carbon tetrachloride. The aqueous solution is slightly alkaline. Melamine is a widely used organic chemical intermediate product with a uniform structure, and its main use is as a raw material for the production of melamine-formaldehyde resin (MF). The melamine-formaldehyde resin raw material is processed and applied to decorative veneers, decorative paints, melamine plasti...

Claims

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Application Information

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IPC IPC(8): G01N21/82
Inventor 胡小松陈芳刘洁戴玉子赵君张慧姜冰李蕾
Owner CHINA AGRI UNIV
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