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Coupling apparatus and technique for fermentation and separation of succinic acid by expanded bed adsorption and in situ extraction

A technology of expanded bed and succinic acid, which is applied in the direction of bioreactor/fermenter combination, fermentation, biochemical cleaning equipment, etc., can solve the problems of fixed bed chromatography column blockage and in-situ separation, and meet the requirements of disinfection and sterilization. Bacteria, meet the effect of pollution-free operation and increase production

Inactive Publication Date: 2009-03-18
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The traditional adsorption process is all carried out in a fixed bed. In situ separation and fermentation of lactic acid bacteria immobilized cells to produce lactic acid (CN200310106657.8) is to enter the fixed bed chromatography column after the fermentation broth is first filtered through a sieve; but most Cell immobilization cannot be carried out in the microbial fermentation process, so that the fermentation liquid carries the cells into the fixed bed chromatography column, which is easy to be blocked, and the in situ separation process cannot be realized

Method used

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  • Coupling apparatus and technique for fermentation and separation of succinic acid by expanded bed adsorption and in situ extraction
  • Coupling apparatus and technique for fermentation and separation of succinic acid by expanded bed adsorption and in situ extraction
  • Coupling apparatus and technique for fermentation and separation of succinic acid by expanded bed adsorption and in situ extraction

Examples

Experimental program
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Effect test

Embodiment 1

[0034] See figure 1 . Expanded bed adsorption coupling device for in-situ extraction of succinic acid fermentation separation, including 1 bioreactor 1, two parallel expanded bed chromatography columns 2, computer control system 3, two transfer pumps 4, 2 parallel first Liquid storage tank 5, four parallel second liquid storage tanks 6, two parallel high level liquid storage tanks 7, five four-way valves 8 and one three-way valve 8.

[0035] There are circulating fluid inlets at the bottom of the two parallel expanded bed chromatography columns, and circulating fluid outlets at the top of the expanded bed chromatography columns;

[0036] 1. The circulating fluid main pipeline forming the circulating fluid circuit, with one three-way valve and four four-way valves on the main pipeline;

[0037] The four ports of the two four-way valves on the circulating fluid main pipeline at the upper part of the expanded bed chromatography column communicate with the circulating fluid outl...

Embodiment 2

[0049] The system of Example 1 was used.

[0050] With the fermentation and separation conditions of Example 1, the fermentation results of the first ten cycles of feeding (including supplementing concentrated sugar solution, nitrogen source and inorganic salt ions) are shown in Table 2. The initial sugar is almost consumed within 72 hours and needs to be supplemented with sugar solution. The consumption of other medium nutrients is not listed here, and other nutrients except concentrated sugar solution can be stored in the high-level liquid storage tank connected to the bioreactor as the feed content.

[0051] Table 2 Fermentation results of ten separation cycles without feeding

[0052]

Embodiment 3

[0054] The system of Example 1 was used.

[0055] The sources and types of expanded bed chromatography column packing resins are shown in the first to third columns of Table 3. The resin was first soaked in deionized water for 24 hours, and then passed through four times of "4wt% HCl, water, 4wt% NaOH and Water" pretreatment, the resin is treated as -OH type, and finally the bed layer is washed with sterile water until the pH value of the effluent is neutral, and the fermentation broth can be prepared for sampling; after reaching the saturated adsorption capacity, select 1wt% ~ 3wt% NaOH or 40wt%-80wt% NaCl is used as eluent for elution. Macroporous adsorption resin or polyvinylpyridine adsorption resin uses 90°C hot water or ethanol as a desorbent, and in this case the pH of the fermentation broth is controlled by adding ammonia water or NaOH. See Table 3 for the effect and acid production of the selected resin after one adsorption, elution, and regeneration cycle of the fer...

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Abstract

The invention belongs to a coupling device and a coupling technology for in situ extraction of succinic acid by an expanded bed and fermentation and separation of the succinic acid, in particular relates to a technology for producing the succinic acid by the fermentation method, a technology for separating the succinic acid by the expanded bed and a fermentation and separation coupling process. The device is to couple a chromatography column of the expanded bed and a succinic acid bioreactor, so as to extract the succinic acid in situ. The device utilizes the characteristic of the chromatography column of the expanded bed that microbial zymotic liquid provided with solid materials is allowed to directly enter the chromatography column, avoids the blockage problem of a chromatography column of a fixed bed due to direct coupling of the fixed bed and the bioreactor in the prior art, saves an additional membrane separator between the bioreactor and the chromatography column of the expanded bed, realizes direct coupling of the chromatography column of the expanded bed and the bioreactor, and performs in situ extraction of target substances and does not cause blockage of the chromatography column of the expanded bed. Moreover, a coupling system for in situ extraction of the succinic acid by the expanded bed and the fermentation and the separation of the succinic acid is established to meet the requirements of sterilization and pollution-free operation.

Description

technical field [0001] The invention belongs to a coupling device and process for in-situ extraction, fermentation and separation of succinic acid by expanded bed adsorption, in particular to a process for producing succinic acid by a fermentation method, a process for separating succinic acid by an expanded bed, and a fermentation, separation and coupling process. technical background [0002] Succinic acid is a platform chemical substance for the synthesis of bulk daily necessities and important chemicals, and has huge economic value. At present, only succinic acid in the food market is produced by fermentation, and the rest are synthesized by petrochemical methods. With the shortage of energy, the prospect of producing succinic acid by biofermentation and biocatalysis is very broad. Succinic acid fermentation is a typical product-inhibiting fermentation process. If the product is not neutralized or separated in time, the fermentation reaction process will not continue. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12P7/46
Inventor 邢建民李强李望良苏志国王丹
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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