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Preparation of 6-O-beta-D- glucosyl-3,6,16,25-tetrahydroxy cycloartane

A technology of cyclopine and tetrahydroxy, which is applied in the field of medicine to achieve the effect of improving the yield

Inactive Publication Date: 2011-08-31
NANJING XIAOZHUANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There has been no report on the preparation of pure compounds by bidirectional fermentation

Method used

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  • Preparation of 6-O-beta-D- glucosyl-3,6,16,25-tetrahydroxy cycloartane
  • Preparation of 6-O-beta-D- glucosyl-3,6,16,25-tetrahydroxy cycloartane
  • Preparation of 6-O-beta-D- glucosyl-3,6,16,25-tetrahydroxy cycloartane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 solid fermentation product:

[0031] Take astragalus dregs, crush them, pass through a 10-mesh sieve, add water to make the water content of astragalus dregs 52%, put them into 10 culture bottles, and sterilize at 120°C for 2 hours; after cooling, inoculate with 10% ganoderma lucidum, and adjust the temperature Ferment at 27°C for 32 days to obtain a solid fermented product; collect the solid fermented product, dry it at 60°C, measure the water content to 8%, and pulverize it.

Embodiment 2

[0032] The preparation of embodiment 2 solid fermentation product:

[0033] Take astragalus dregs, crush them, pass through a 10-mesh sieve, add water to make the water content of astragalus dregs 45%, put them into 10 culture bottles, and sterilize at 121°C for 1.5 hours; after cooling, inoculate with 5% ganoderma lucidum, and adjust the temperature Ferment at 25°C for 45 days to obtain a solid fermented product; collect the solid fermented product, dry it at 50°C, and pulverize it with a water content of 10%.

Embodiment 3

[0034] The preparation of embodiment 3 solid fermentation product:

[0035] Take astragalus dregs, crush them, pass through a 10-mesh sieve, add water to make the water content of astragalus dregs 60%, put them into 10 culture bottles, and sterilize them at 121°C for 2 hours; It is adjusted to 30°C and fermented for 40 days to obtain solid fermented product; the solid fermented product is collected, dried at 70°C, and pulverized with a water content of 6%.

[0036] The dried pulverized solid fermented product obtained in Example 1 is extracted and separated:

[0037] Take 1 kg of solid fermented product powder, add 70% ethanol extract to reflux extraction twice, each time for 2 hours, combine the 2 extracts, recover ethanol under reduced pressure to a thick paste, and obtain extract. Add an appropriate amount of water to dissolve the extract, shake and extract the aqueous solution for 3 times with n-butanol saturated with water, combine the n-butanol solution, wash fully with...

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Abstract

The invention discloses a method for preparing 6-O-Beta-D-glucosyl-3,6,16,25-tetrahydroxycycloartane. The method comprises the following steps: inoculating pharmaceutical fungi in a solid culture medium containing Chinese traditional medicinal materials or residue thereof; fermenting under a certain fermentation condition; collecting the solid fermented material; and drying, pulverizing and extracting and separating to obtain the product. The method has the advantages that the process is simple, the waste reutilization is implemented, during the extracting and separating process, the macro porous resin method is not adopted to enrich the compound so that the repeated activation of the macro porous resin is obviated, the mixed n-butyl alcohol extraction solution is washed with sodium hydroxide solution to remove a great amount of phenolic acid impurities and color pigments, a neutral alumina chromatographic column is adopted for the purification so that the pigment impurities with the polarity similar to that of the target component is effectively removed so that the pure product can be directly obtained without need of the re-crystallization, the yield of the pure product is improved, and the method is worth extending and applying..

Description

technical field [0001] The invention relates to a preparation method of 6-O-β-D-glucosyl-3,6,16,25-tetrahydroxycyclopine, belonging to the technical field of medicine. Background technique [0002] Compound 6-O-β-D-glucosyl-3,6,16,25-tetrahydroxycyclopine (hereinafter referred to as compound 1), see chemical formula (I): [0003] [0004] Compound 1 can improve the body's immunity. According to literature reports, under the stimulation of PHA, it can significantly induce the activity of IL-2, and the increase rate is as high as 80.9%, while the control group is only 51.1%. Under the stimulation of LPS, it can induce IL-2 activity. -1β and IL-8 activity [Yesilada, E, Bedir, E, Calis, I, Takaishi, Y, Ohmoto, Y. Effects of triterpene saponins from Astragalus species on invitro cytokine release. J. Ethnopharmacology 2005; 96: 71-77] . IL-2 is a cytokine activated by T cells, which has significant immune regulation and anti-tumor effects; IL-1β can activate T cells and macro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J53/00C12P19/56C12R1/645
Inventor 刘维周阮鸣张李阳陈玉胜饶玉鹏霍光明
Owner NANJING XIAOZHUANG UNIV
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