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Method for rapid quantitatively evaluating interaction of medicament nucleic acids

A quantitative evaluation and drug technology, applied in the fields of chemistry and physical sciences, can solve the problems of time-consuming, high equipment, etc., and achieve the effects of simple operation, less sample consumption, huge scientific value and economic value

Inactive Publication Date: 2009-02-25
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Generally speaking, simple and fast methods cannot obtain higher information content, while methods with relatively large information content are time-consuming and require high instrumentation.

Method used

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  • Method for rapid quantitatively evaluating interaction of medicament nucleic acids
  • Method for rapid quantitatively evaluating interaction of medicament nucleic acids
  • Method for rapid quantitatively evaluating interaction of medicament nucleic acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Assay for fluorescently labeled nucleic acids. The chips used in this experiment are figure 1 Shown is a glass chip containing four electrophoretic cells with channels 50 μm wide and 15 μm deep. Put the buffer into the buffer reservoir (4), and apply negative pressure to fill the buffer with the separation channel (5) and the waste liquid pool (6), mix 10 μM fluorescently labeled nucleic acid with 10 μM internal standard fluorescein, and mix the four Parts of the sample are added to the sample cell (2). Apply a voltage (field strength 400V) between the sample cell (2) and the waste liquid cell (6) for 6 seconds, then switch the voltage to between the buffer solution reservoir (4) and the waste liquid cell (6) (field strength 400V) strong 400V), the sample cell (2) applies a suppression voltage to prevent sample leakage, and is detected at a distance of 3cm from the injection point (results see figure 2 ). It can be seen from the figure that the peak area ratios of ...

Embodiment 2

[0028] Determination of the binding constant of netropsin and nucleic acid. The chips used in this experiment are figure 1 Shown is a glass chip containing four electrophoretic cells with channels 50 μm wide and 15 μm deep. Put the buffer into the buffer reservoir (4), and apply negative pressure to fill the buffer with the separation channel (5) and the waste liquid pool (6), and mix the netlastin and the fluorescently labeled nucleic acid at a ratio of 0.5:1-2: 1 Concentration ratio mixing (10 μM internal standard fluorescein is added), and four mixed solutions with different concentration ratios are added to the sample pool (2). Apply a voltage (field strength 400V) between the sample cell (2) and the waste liquid cell (6) for 6 seconds, then switch the voltage to between the buffer solution reservoir (4) and the waste liquid cell (6) (field strength 400V) strong 400V), the sample cell (2) applies a suppression voltage to prevent sample leakage, and is detected at a dista...

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Abstract

A fast quantitative evaluation method upon interaction of drug nucleic acids is characterized in that the interaction of drug nucleic acids with dedicated array micro-fluidic chip is quantitatively evaluated. Compared with the prior art, the invention has the advantages of direct, fast and simple operation, low sample consumption, and wide application in high flux screening of nucleic acid target drugs.

Description

Technical field: [0001] The invention belongs to the science of chemistry and physics, and specifically relates to chip electrophoresis technology; in particular, it provides a method for quantitatively evaluating drug-nucleic acid interaction using a special array microfluidic chip. Background technique: [0002] Nucleic acid molecules are polymorphic (including sequence, structure, etc.), and their structures often contain specific sites as the targets of some drugs. The interaction of some drugs with nucleic acids can lead to cleavage of nucleic acids or distortion of the spatial helical structure. And many of these nucleic acid-drug interactions can show biological and clinical pharmacological effects, such as: inhibiting tumor cell growth, virus, bacteria, fungal and parasitic infections, etc. Design, developmental research and clinical medication have extremely important guiding significance. Although many drugs based on nucleic acid targets have been used in clinical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N27/26
Inventor 林炳承沈铮秦建华戴忠鹏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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