Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Effective component of Sinomenium acutum and preparation and use thereof

An effective component, a technology for preparing liquid phase, which is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve problems such as side reactions and involvement of normal cells.

Inactive Publication Date: 2009-01-21
TIANJIN TASLY PHARMA CO LTD
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Effective component of Sinomenium acutum and preparation and use thereof
  • Effective component of Sinomenium acutum and preparation and use thereof
  • Effective component of Sinomenium acutum and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0151] The preparation of the effective component of embodiment 1 Fangji

[0152] Take 250 g of Antichristia chinensis medicinal material, grind it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. Concentrate the extract into extract to obtain 8.6g, dissolve and load the sample with ethanol, and pass through the ODS-C18 column. First, use 1250ml of 30% ethanol as the mobile phase to obtain the eluate, discard it, and then replace it with 1250ml of 95% ethanol as the mobile phase. phase, to obtain the eluent II, and the eluent II was concentrated and dried to obtain a sample; the sample obtained by further separation by preparative liquid chromatography; the separation conditions of preparative chromatography: the chromatographic column is a preparative column Zorbax SB-C18; 21.2mm×250mm , the mobile phase is water A and acetonitrile B, and the gradient elution procedure is as follows:

[0153] ...

Embodiment 2

[0155] The analysis of embodiment 2 Fangji effective components

[0156] Combined HPLC-ELSD analysis of the effective components of Fangji in Example 1

[0157] Chromatographic conditionsChromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glacial acetic acid in w...

Embodiment 3

[0160] Embodiment 3 Fangji effective component preparation

[0161] Take the effective component of Fangji in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, after the material is changed, move it to drip irrigation, and drop the drug solution into liquid paraffin at 6-8°C, remove Oil, made 400 drop pills.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an effective component of radix stephaniae tetrandrae, a preparation method and application thereof; the preparation process of the effective component of radix stephaniae tetrandrae in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting radix stephaniae tetrandrae; step 2, the extract is processed with chromatographic column chromatography to obtain eluate; step3, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 8.0-16.0 min is collected to obtain the effective component.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Fangji, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained from plants with...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/59A61P35/00C07D221/06C07D455/06A61K125/00
Inventor 贺庆刘雳史强程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com