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Effective component of Sinomenium acutum and preparation and use thereof

A technology for effective components and self-prevention, which can be used in medical preparations containing active components, organic chemistry, drug combinations, etc., and can solve problems such as side reactions and involvement of normal cells.

Inactive Publication Date: 2009-01-21
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of Sinomenium acutum and preparation and use thereof
  • Effective component of Sinomenium acutum and preparation and use thereof
  • Effective component of Sinomenium acutum and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0124] The preparation of the effective component of embodiment 1 Fangji

[0125] Crush 250g of Fangji, add 8 times the amount of ethyl acetate: ethanol = 1:1, heat and reflux for 1 hour, extract twice, add 8 times the amount of 70% ethanol to the dregs, heat and reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract After concentrating the extract, dissolve the sample with 5% methanol and pass it through the ODS-C18 column. First, use 5% methanol as the mobile phase, and then replace it with 60% methanol as the mobile phase to obtain the eluent, which is used to prepare the liquid phase Chromatography continues to separate the eluent, separation conditions: the chromatographic column is an Agilent preparative column (Zorbax SB-C18; 21.2mm×250mm), the mobile phase is water A and acetonitrile B, and the gradient elution procedure is as follows

[0126]

[0127] The flow rate is 10ml / min, the column temperature is room temperature; the sample is dis...

Embodiment 2

[0128] The analysis of embodiment 2 Fangji effective components

[0129] Combined HPLC-ELSD analysis of the effective components of Fangji in Example 1

[0130] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glacial acetic acid in ...

Embodiment 3

[0133]Embodiment 3 Fangji effective component preparation

[0134] Take any effective component of Fangji in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, move the material to the drip irrigation after the material, and drop the medicine liquid to 6-8°C liquid paraffin In the process of removing oil, 400 dropping pills were prepared.

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PUM

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Abstract

The invention relates to an effective component of radix stephaniae tetrandrae, a preparation method and application thereof; the preparation process of the effective component of radix stephaniae tetrandrae in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting radix stephaniae tetrandrae; step 2, herb residue is extracted with ethanol to obtain extract; step 3, the extract is processed with chromatographic column chromatography to obtain eluate; step4, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 14.1-17.8 min, 17.8-21.0 min, 21.0-24.5 min, 24.5-27.7 min, 27.7-30.5 min, 24.5-27.7 min, 34.2-41.0 min and 41.0-45.0 min is collected to obtain the effective component.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Fangji, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained from plants with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/59A61P35/00C07C69/734C07D455/06C07D321/00C07D221/18C07D491/056C07D491/18A61K125/00
Inventor 贺庆刘雳史强程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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