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Method for on-line detection for solid-state biomass bioconversion procedure

A technology for biotransformation and biomass, applied in the field of instrument analysis, to achieve good economic benefits, applicability, and ease of use

Inactive Publication Date: 2009-01-07
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the existing technology is difficult to quickly determine the composition of complex solid matrix on-line, make full use of the rich information contained in the near-infrared spectrum, and provide an on-line, fast, easy-to-operate, non-destructive determination of insoluble biomass Method for Transforming Process Parameters

Method used

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  • Method for on-line detection for solid-state biomass bioconversion procedure
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  • Method for on-line detection for solid-state biomass bioconversion procedure

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Embodiment 1

[0016] Embodiment 1 uses near-infrared spectroscopy to quickly measure the water content of the medium in the solid-state fermentation process

[0017] The flow process of the embodiment of the present invention is as follows:

[0018] The first step is the preparation of the modeling sample set, using steam-exploded wheat straw and wheat bran as the main medium for solid-state fermentation, adjusting the medium to 45-85%, and inoculating Trichoderma viride at 30°C after the medium is sterilized Solid-state fermentation is carried out in a gas-phase double-dynamic solid-state fermentation reactor. After a certain period of cultivation, it is used as a calibration set sample for a near-infrared multiple regression prediction model, and the fermentation sample is taken out. The number of samples is preferably 50, and the representativeness of the sample set has a great influence on the stability and adaptability of the prediction model. It is better to require the standard devi...

Embodiment 2

[0024] Example 2 Rapid Determination of the Biomass of Medium in Solid State Fermentation Process with Near Infrared Spectroscopy

[0025] The flow process of the embodiment of the present invention is as follows:

[0026] The first step is the preparation of the modeling sample set. Steam-exploded corn stalks and wheat bran are used as the main medium for solid-state fermentation. The water content of the medium is adjusted to about 75%. After the medium is sterilized, it is inoculated with Penicillium decumbens at 30°C Carry out solid-state fermentation, and after cultivating for a certain period of time, take out the fermentation sample and use it as the calibration set sample of the near-infrared multiple regression prediction model. The number of samples is preferably 50.

[0027] The second step is the determination of the near-infrared spectrum of the solid-state fermentation sample. This embodiment adopts the Nexus type Fourier transform near-infrared spectrometer pr...

Embodiment 3

[0032] Example 3 Rapid Determination of Cellulase in Medium in Solid-state Fermentation Process by Near Infrared Spectroscopy

[0033] The flow process of the embodiment of the present invention is as follows:

[0034] The first step is the preparation of the modeling sample set. Steam-exploded corn stalks are used as the main medium for solid-state fermentation, and the water content of the medium is adjusted to about 75%. After the medium is sterilized, Trichoderma viride is inoculated for solid-state fermentation at 30°C. After culturing for a certain period of time, the fermentation samples were taken out and used as calibration set samples for the near-infrared multiple regression prediction model. The number of samples is preferably 50.

[0035] The second step is the determination of the near-infrared spectrum of the solid-state fermentation sample. This embodiment adopts the Nexus type Fourier transform near-infrared spectrometer produced by Nicolet Company of the Un...

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Abstract

The invention relates to a method for on line detecting biological transmutation process of solid-state biomass, the steps of the method are as follows: choosing not more than 50 portions of samples of representative biomass in the biological transmutation process as standard samples for acquiring standard maps; obtaining the parameters on target process by other means; establishing a quantitative model through multiple regression to quantify other unknown processes. The method provided by the invention can realize on-line detection of parameters in biological transmutation process only by establishing a forecasting model and can automatically detect and control the biological transmutation process, without sample preparation or reagent consumption. The method is used for forecasting related coefficients in solid-state fermentation, including moisture content, biomass, grease output and cellulase, the forecast related coefficients respectively reach 0.994, 0.999, 0.984 and 0.994.

Description

technical field [0001] The invention relates to the field of instrumental analysis, in particular to a non-destructive, on-line method for determining the content of substrates and products in the solid biomass biotransformation process. Background technique [0002] In the process of biomass conversion, biotransformation has become a research hotspot because of its friendliness to the environment. The bioconversion of biomass mainly includes microbial fermentation (mainly solid-state fermentation) and enzymatic hydrolysis. The substrates of traditional solid-state fermentation and modern solid-state fermentation are mainly agricultural and sideline products. Commonly used fermentation substrates include corn straw, wheat straw, rice straw, bran, rice husk, corn cob, corn, millet, soybean, bagasse , cassava, etc. From the source analysis, straw-based biomass is of course the main substrate of solid-state fermentation, especially the bulk products produced by solid-state fe...

Claims

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Application Information

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IPC IPC(8): G01N33/48G01N21/47
Inventor 陈洪章李宏强
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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