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Swine blood bovine and method for preparing same

A technology of iron porphyrin and pig blood, applied in the field of nutritional health care products and preparation thereof, can solve the problems of insoluble iron porphyrin, affecting absorption and utilization, and no breakthrough progress in the solubility of iron porphyrin.

Inactive Publication Date: 2009-01-07
HEFEI UNIV OF TECH
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  • Abstract
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Problems solved by technology

The porphyrin iron extracted from red blood cells is non-toxic and harmless, and is generally not affected by dietary factors. It is expected to replace the currently commonly used iron supplements. water, seriously affecting its absorption and utilization
Some scholars and experts at home and abroad attempt to improve the solubility of porphyrin iron through different extraction methods of porphyrin iron. However, there are problems such as process flow, cost, product purity, yield and solubility, especially the solubility of porphyrin iron. get a breakthrough

Method used

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  • Swine blood bovine and method for preparing same
  • Swine blood bovine and method for preparing same
  • Swine blood bovine and method for preparing same

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Effect test

Embodiment Construction

[0018] 1. Extract and separate red blood cells from fresh pig blood

[0019] Collect fresh pig blood, add trisodium citrate with a concentration of 0.38% (w / w) as an anticoagulant, and add 4% (v / v) of the pig blood volume, centrifuge at 4000r / min for 15min, and collect red blood cells After the wall was broken by ultrasound, chloroform was added in an amount of 0.15 times (v / v) of the amount of red blood cells, centrifuged at 4000r / min for 15min, the upper hemoglobin solution was collected, and refrigerated at 4°C for later use.

[0020] 2. Enzymolysis

[0021] Take the hemoglobin solution that is refrigerated and add alkaline protease to enzymolyze it for 9 hours at 35°C to obtain an enzymatic hydrolysis solution. The amount of enzyme added is 8000U / g in terms of hemoglobin.

[0022] 3. Post-processing

[0023] Add acetone to the enzymolysis solution, and adjust the pH to 3.0 with acetic acid. The amount of acetone added is 4 to 6 times the amount of red blood cells (v / v). ...

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Abstract

Pig-blood porphyrin iron is porphyrin iron that is obtained from fresh pig blood through separation, extraction and medication. A preparation method thereof comprises the following steps: fresh pig blood is used as raw material, and serum is removed through separation to extract hematoglobin; hematoglobin is processed through enzymolysis by using proteinase after ultrasonic impingement; porphyrin iron is separated and extracted through an acidic acetone method after enzymolysis. In the preparation method, porphyrin iron of 3.12g can be extracted from 1 liter fresh pig blood, the iron content is 9.1 percent, the solubility limit is increased by 65.5 percent compared with a standard substance, and pig-blood porphyrin iron is expected to replace the common iron supplementary at present.

Description

1. Technical field [0001] The invention relates to a nutritional health product and a preparation method thereof, in particular to an iron supplement and a preparation method thereof, in particular to a porcine hematoporphyrin iron and a preparation method thereof. 2. Background technology [0002] The currently commonly used iron supplements are non-porphyrin iron, such as ferrous sulfate, which is an inorganic element iron, which has a low absorption rate in the human body and has certain side effects. The iron content of products made from pig blood on the market is only 1.4%, and the iron in the form of organic heme is even less. The porphyrin iron extracted from red blood cells is non-toxic and harmless, and is generally not affected by dietary factors. It is expected to replace the currently commonly used iron supplements. Water seriously affects its absorption and utilization. Some scholars and experts at home and abroad attempt to improve the solubility of porphyri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22C07F15/02C12P17/18C12P9/00
Inventor 范远景姬莹莹冯铨祥余丽明刘盛扬陈嘉可郑琛庞伟
Owner HEFEI UNIV OF TECH
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