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Anti-opioid peptide, antagonistic peptide thereof and use thereof

A technology of anti-opioid peptides and antagonistic peptides, applied in the fields of application, medical preparations containing active ingredients, peptide sources, etc., can solve the problems of acupuncture tolerance and limited efficacy of acupuncture analgesia

Inactive Publication Date: 2008-12-17
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Professor Han Jisheng used an acupuncture treatment device to treat withdrawal symptoms, trying to stimulate the synthesis of endogenous opioids in the body through specific frequency electrical pulse stimulation to relieve symptoms, but its biggest defect is that the effectiveness of acupuncture analgesia in the population is limited and continues Acupuncture also has tolerance issues

Method used

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  • Anti-opioid peptide, antagonistic peptide thereof and use thereof
  • Anti-opioid peptide, antagonistic peptide thereof and use thereof
  • Anti-opioid peptide, antagonistic peptide thereof and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1. Obtainment of anti-opioid peptide 99A and its functional verification

[0085] 1. Obtainment of porcine brain anti-opioid peptide 99A and detection of its dose-dependent effect

[0086] 1. Discovery of anti-opioid peptides

[0087] In the isolated mouse vas deferens specimens, stimulation with a square wave pulse (0.1MS width, 1 time / second) can cause contraction (see the literature for the specific method: J. Hughes et al (1975) Effect of morphine on adrenergic transmission in the Mouse Vas deferens.Assessment of agonist and antagonistpotencies of Narcotic analgesics 53,371-381), then, successively add morphine hydrochloride (3.2nM) or dog brain extract (AOS, 200μg / mL) (for dog brain extract The concentrated solution was successively filtered through SephadexG-50 gel (purchased from Pharmacia Company), and after cation exchange chromatography of carboxymethyl-cellulose (CM-C was purchased from H.Reeve-Angel&.Co.Ltd Company), the sample was obtained), To ob...

Embodiment 2

[0116]Example 2. Obtaining and Activity Detection of Anti-opioid Peptide 99A Antagonist Peptide

[0117] 1. Obtaining antagonistic peptides of 99A-16, 99A-19 and DBI-16

[0118] According to the principle of interaction between protein molecules, the reference (J.R.Heal et al Specific interactions between sense and complementary peptides: The Basis for the proteomic code ChemBIOChem 2002, 3, 114-131) designed 99A-16, 99A-19 and DBI -16 antagonistic peptides of three short peptides, wherein the antagonistic peptide of 99A-16 is named CP-99A-16+5, has the amino acid residue sequence of SEQ ID NO: 5, consists of 21 amino acid residues, and The coding sequence has the nucleotide sequence of SEQ ID NO: 8 in the sequence table, consisting of 63 bases; the antagonistic peptide of 99A-19 is named CP-99A-19, and has the amino acid residue sequence of SEQ ID NO: 6 , consisting of 19 amino acid residues, its coding sequence has the nucleotide sequence of SEQ ID NO: 9 in the sequence tab...

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Abstract

The invention discloses an anti-opiate peptide, an antagonistic peptide and an application thereof. The anti-opiate peptide is one of amino-acid residue sequences as follows: 1) SEQ ID NO: 1 in the sequence table; 2) substitution and deletion of one to ten amino-acid residues, or addition of polypeptide which has the functions of antagonism for morphine and antinociception is carried out on the amino-acid residue sequence of SEQ ID NO:1 in the sequence table. The antagonistic peptide is one of the following amino-acid residue sequences: 1) SEQ ID NO: 5-7 in the sequence table; 2) substitution and deletion of one to ten amino-acid residues, or addition of polypeptide which can enhance the analgesic effect of the morphine, reverse the tolerance of the morphine and / or weaken or eliminate the effect of abstinence symptoms is carried out on the amino-acid residue sequence of SEQ ID NO: 5-7 in the sequence table. The anti-opiate peptide, the active fragment thereof and the antagonistic peptide of the invention can play an important role in the preparation fields of medicine and rehabilitation drugs and have wide application prospect.

Description

technical field [0001] The present invention relates to peptides and their coding genes and applications, in particular to an anti-opioid peptide and its antagonistic peptide which can antagonize the antinociceptive effect of morphine, and the antagonistic peptide can enhance morphine analgesia, reverse morphine tolerance and / or Use in drugs that reduce or eliminate the effects of withdrawal symptoms. Background technique [0002] As we all know, rampant drug trafficking and drug use around the world has become a cancer that endangers society, seriously interfering with orderly economic construction and social stability. In addition, intravenous drug use has also caused a rise in the infection rate of AIDS populations. At present, although the governments of various countries have stepped up efforts to jointly crack down on drug smuggling, drug abuse and drug trafficking activities are still repeatedly banned. One of the important reasons is that the principles of morphine t...

Claims

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Application Information

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IPC IPC(8): C07K14/435A61K38/17A61P25/36C12N15/12
Inventor 陈玉珍吴才宏曲红
Owner PEKING UNIV
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