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Real-time PCR system

A technology of polymerase and chain reaction, which is applied in the direction of laboratory equipment, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of uncontrollable amplification level, infeasible comprehensive analysis, and inability to analyze together at the same time, so as to achieve high precision The effect of control

Inactive Publication Date: 2008-11-12
SONY CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, they come with the problem of not being able to analyze many samples together at the same time, therefore, comprehensive analysis is not feasible
Therefore, there is a problem that the amplification level of each sample cannot be controlled so as to be equal to each other and by-products may appear

Method used

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Embodiment Construction

[0033] Hereinafter, preferred embodiments of the real-time PCR system according to the present invention are described with reference to the accompanying drawings. It should be noted that the drawings show preferred embodiments of the invention by way of example, and the invention should not be construed narrowly by the preferred embodiments.

[0034] First, refer to figure 1 , the first embodiment of the real-time PCR system according to the present invention will be described hereinafter.

[0035] figure 1 Reference number 1 in indicates the first embodiment of the real-time PCR according to the present invention. As desired, the size and layer structure of the real-time PCR system 1 can be selectively determined according to the purpose of application. As long as the real-time PCR system 1 can achieve the purpose of the present invention, the configuration of the real-time PCR system 1 can also be designed or improved as required.

[0036] The real-time PCR system 1 is ...

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Abstract

A real-time PCR system for detecting gene expression levels includes plural reaction regions, a like plural number of heating portions arranged corresponding to the reaction regions and having heat sources, respectively, an optical unit capable of irradiating exciting light of a specific wavelength to all of the plural reaction regions, and a like plural number of fluorescence detecting portions (15) arranged corresponding to the reaction regions, respectively. The heating portions are each provided with a temperature detector for detecting a temperature in a vicinity of the corresponding heat source and converting the temperature into an electrical signal and also with a controller for controlling a thermal dose from the corresponding heat source based on a correlation between electricalsignals and calorific values of the heat source stored beforehand.

Description

[0001] Cross References to Related Applications [0002] The present invention contains subject matter related to Japanese Patent Application JP 2007-126344 filed in the Japan Patent Office on May 11, 2007, the entire content of which is hereby incorporated by reference. technical field [0003] The invention relates to a real-time PCR system. More specifically, the present invention relates to a real-time PCR system for analyzing gene expression and the like. Background technique [0004] In recent years, hybridization detection techniques arising from DNA chips and DNA microarrays have found more and more practical applications. A DNA chip includes a wide variety of DNA probes integrally immobilized on the surface of a substrate. By using this DNA chip to detect hybridization on the surface of a substrate in the DNA chip, gene expression in cells, tissues, and the like can be comprehensively analyzed. [0005] This has now become a standard method for the quantitative a...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/38C12Q1/68
CPCB01L2300/0654G01N21/6428B01J2219/00853B01L2300/0829B01L2300/0893B01L7/52G01K7/015B01L2300/0627B01J2219/00783B01L2300/1827B01J2219/00873
Inventor 森胁俊贵世取山翼濑川雄司
Owner SONY CORP
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