Method for transforming root cell of Cymbidium

A technology of cell transformation and orchid, applied in horticultural methods, botanical equipment and methods, preparation of organic fertilizers, etc., can solve problems such as waste of resources, failure to succeed, etc., achieve convenient access to materials, broaden basic content and development fields, Idea Advanced Effects

Inactive Publication Date: 2008-10-08
刘开宁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When orchids are repotted every year, due to the traditional concept that orchid roots have no natural regeneration ability, tens of thousands of broken roots of precious orchids are discarded all over the country, resulting in a huge waste of resources.
Although experts, scholars and orchid growers have been trying to find ways to use discarded orchid roots to grow seedlings under natural conditions for many years, they have failed for various reasons.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of plant materials: crush 10 liters of willow bark, 7 liters of pine bark, 20 liters of qinggang bark, 1 liter of qinggang leaves, 1 liter of ginkgo leaves, 1 liter of eucalyptus leaves, 10 liters of dry sea grass charcoal, and 7 liters of peat soil If the size is less than 2 cm, soak it in water for 1 month, take it out, and ferment it naturally for more than 1 year, then mix in 10 liters of yellow sand, 8 liters of fire-fired hollow soil, and 10 liters of sea pumice, and soak in boiling water for 2 hours to remove stains Finally, dry it in the sun and store it in a sealed container. After 5 days, spray the plant material with 1:800 times Tuobu fluid and 1:1000 times glucose solution, and stack it naturally for later use;

[0033] 2. Treatment of waste orchid roots: soak the waste orchid roots in 1:800 times thiophanate dilution solution for disinfection, take out after 30 minutes of disinfection and rinse with clean water to wash off the residual drug on...

Embodiment 2

[0038] The basic process is the same as in Example 1, the difference is that in the preparation process of the plant material, the plant material components are 7 liters of willow bark, 4 liters of pine bark, 18 liters of bark of Qinggang, 1 liter of leaves of Qinggang, 1 liter of ginkgo leaves, eucalyptus 1 liter of leaves, 10 liters of dry sea grass charcoal, 5 liters of peat soil particles, 12 liters of yellow sand, 8 liters of fire-burned hollow soil, and 8 liters of sea pumice. 1:1500 times of glucose solution; during the processing of waste orchid roots, the disinfectant is 1:1000 times of Tuobujin solution, and the nutrient is 1:1500 times of glucose solution and 1:2500 times of salicylic acid Mixed solution of acid solution; during the mutagenesis process of rhizosphere cells, the disinfectant solution is 1:1000 times of Tuobujin solution, and the nutrient solution used for spraying orchid strains and basin surfaces when implanting discarded orchid roots is 1:2000 time...

Embodiment 3

[0040] The basic process is the same as in Example 1, the difference is: in the preparation of the plant material, the components of the plant material are 15 liters of willow bark, 10 liters of pine bark, 25 liters of bark of Qinggang, 2 liters of leaves of Qinggang, 1 liter of ginkgo leaves, eucalyptus 2 liters of leaves, 25 liters of dry seaweed charcoal, 10 liters of peat soil, 5 liters of yellow sand, 2 liters of fire-burned hollow soil, and 2 liters of sea pumice. The disinfectant and nutrient are respectively 1:900 times chlorothalonium and 1: 1200 times of chlorothalonium solution; in the process of waste orchid roots, the disinfectant is 1: 900 times of chlorothalonium solution, and the nutrient is 1: 1200 times of chlorothalonium solution and 1: 2800 times the mixed solution of salicylic acid solution; in the mutagenesis process of rhizosphere cells, the disinfectant solution is 1:900 times chlorothalonil solution, which is used for spraying orchid strains and basin s...

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PUM

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Abstract

The invention provides a method for transforming the rhizosphere cells of Chinese orchid; the method utilizes organic planting material for inducing the rhizosphere cells of Chinese orchid to be transformed from nutrition type cells to embryonic type cells under natural condition. The method includes the following steps: (1) the preparation of the planting material; (2) the disposal of deserted orchid root; (3) the mutation of the rhizosphere cells; (4) material replacement and transplantation for a new seedling. The orchid seedling cultivated by the method of the invention has strong anti-adversity ability, can comprehensively guarantee the accuracy and entireness of gene inheritance; the sprout, seedling formation and flower bearing of the orchid seedling are identical with that of the maternal natural plant. Meanwhile, the method follows nature, which has advanced concept, convenient material taking and simple operation, thus having important significance for protecting the Chinese orchid resource, developing precious varieties and facilitating Chinese orchid to enter numerous households in advance.

Description

technical field [0001] The invention relates to a method for transforming rhizosphere cells of Chinese orchid, specifically, a method for using organic plant materials to induce the transformation of rhizosphere cells of Chinese orchid from vegetative cells to embryonic cells under natural conditions. Background technique [0002] Orchidaceae is a high-level ornamental plant that has been cared for by humans for thousands of years. China is a big country producing orchids, and tens of millions of people are engaged in orchid cultivation. With the continuous rise of the orchid industry, wild orchid resources are becoming less and less, and individual species have developed to the verge of extinction. And every year when orchid ramets are repotted, due to the influence of the traditional concept that orchid roots have no natural regeneration ability, tens of thousands of broken roots of valuable orchids are discarded all over the country, resulting in a huge waste of resource...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01G1/00C05F17/00
CPCY02W30/40
Inventor 刘开宁蔡云飞
Owner 刘开宁
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