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Screening preparation for superior enterococcus faecalis and application thereof

A technology of Enterococcus faecalis and screening method, which is applied in the field of screening and preparation of excellent Enterococcus faecalis to achieve the effects of fast growth, short fermentation period and strong vitality

Inactive Publication Date: 2008-10-01
孙卫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no literature report that E. faecalis metabolites have certain pharmacological effects.

Method used

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  • Screening preparation for superior enterococcus faecalis and application thereof
  • Screening preparation for superior enterococcus faecalis and application thereof
  • Screening preparation for superior enterococcus faecalis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] specific implementation plan

[0028] 1. Breeding of strains

[0029] Samples were collected from the intestinal tract of healthy people, spread on blood plates, and cultured at 37°C for 18-24 hours. Colonies without hemolytic patterns around the colonies were picked. After microscopic observation confirmed that they were Enterococcus faecalis, single colonies were used to isolate and purify. A series of Enterococcus faecalis isolated were cultured in the following medium: glucose 0.5%, tryptone 0.4%, peptone 0.6%, yeast extract 0.3%, beef extract 0.5%, sodium chloride 0.5%, water 100ml, pH7. 2. After standing at 37°C for three days, centrifuge, superimpose the supernatant or its dilution in a precipitation tube containing a certain amount of antiserum, and observe the appearance and thickness of the precipitation ring within a certain period of time to judge the polypeptide produced by the strain Ability to select strains with strong ability to produce polypeptides. ...

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Abstract

The present invention provides a screen preparing method of excellent enterococcus faecalis and its application. Enterococcus faecalis is the most common representative species in the Enterococcus, living in human intestinal, having no hazard to human, and having benefits. But Enterococcus faecali arises server infection some time, and having resistance to antibiotics. The invention repeatedly cultures and screens enterococcus faecalis, selecting out a strain which has exuberant life, quick growth speed, high fermentation unit, short fermentation cycle, high content of fermentation product, strength production adaptability as a producing strain by researching the contents of morphological characteristics, culture characteristics, physiological and biochemical response, metabolites, genetic character and protein expression, genetic stability, pilot producing index etc, forming a scale production. The broth has functions of immunity adjustment, antitumor etc.

Description

technical field [0001] The invention relates to a method for screening and preparing excellent Enterococcus faecalis and its application. Background technique [0002] Microorganisms are a type of tiny organisms that are closely related to human beings. They have a variety of species and complex functions. Some microorganisms play a very important role in the development of drugs. The main link of microbial drug development includes the selection of strains. Fermentation, optimization of fermentation process, research on extraction and refining process, etc. [0003] As early as the 19th century, it was discovered that the tumors of cancer patients infected by hemolytic streptococcus naturally subsided. People have successively discovered that many microorganisms and their preparations have certain pharmacological activities. [0004] Enterococcus was separated from Streptococcus in 1984 and became an independent new genus. According to the DNA homology, it was found that E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12N1/20A61K35/74A61P1/00A61P3/04A61P3/06A61P7/06A61P9/12A61P11/04A61P15/14A61P17/10A61P17/16A61P19/08A61P25/20A61P27/02A61P29/00A61P35/00A61P37/04A61P39/02A61P39/06A61K35/744
Inventor 孙卫
Owner 孙卫
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