Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Enzyme catalysis electricity-conducting immunity sensor based on micro-gap array electrode and its method for detecting infective virus

An immune sensor and array electrode technology, applied in the field of biological analysis, can solve the problems of inaccurate quantification and low sensitivity, and achieve the effect of portable price, high sensitivity and simple detection steps

Inactive Publication Date: 2008-09-24
HUNAN UNIV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to propose an enzyme-catalyzed conductance immunosensor based on micro-gap array electrodes and a method for detecting infectious diseases in order to overcome the shortcomings of traditional infectious disease immunodetection methods such as low sensitivity and inaccurate quantification. It is characterized by high sensitivity, rapidity and low cost for detecting infectious diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzyme catalysis electricity-conducting immunity sensor based on micro-gap array electrode and its method for detecting infective virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: The immunosensor detection human immunodeficiency virus (HIV) antibody based on microgap array electrode

[0025] 1. Preparation of immunosensor:

[0026] After washing the microgap array gold electrode with absolute ethanol (100%) three times (1 min each time), the electrode was immersed in 1 M NaOH solution for 30 min; then the electrode was washed three times with ultrapure water (1 min each time), and dried Then, the electrode is immersed in the ethanol solution containing aminopropyltrimethoxysilane 5% (volume percentage), and left to stand at room temperature for 24 hours; after being washed three times with ultrapure water, it is dried to obtain a silanized micro-gap array gold electrode.

[0027] Immerse the silanized micro-gap array gold electrode in glutaraldehyde aqueous solution (5% mass fraction), and let it stand at room temperature for 1 hour; after washing with ultrapure water three times, add 30 μL genetically recombined HIV-1+2 dropwise ...

Embodiment 2

[0031] Embodiment 2: Detection of hepatitis B virus surface antigen (HBsAg) by immunosensor based on micro-gap array electrodes

[0032] 1. Preparation of immunosensor:

[0033] After washing the microgap array gold electrode with absolute ethanol (100%) three times (1 min each time), the electrode was immersed in 1M NaOH solution for 30 min; then the electrode was washed with ultrapure water three times (1 min each time), and dried; Then, the electrode is immersed in an ethanol solution containing 5% (volume percentage) of aminopropyltrimethoxysilane, and left to stand at room temperature for 24 hours; it is washed with ultrapure water three times and dried to obtain a silanized micro-gap array gold electrode .

[0034] Immerse the silanized micro-gap array gold electrode in glutaraldehyde aqueous solution (5% mass fraction), and let it stand at room temperature for 1 hour; after washing with ultrapure water three times, add 30 μ L of hepatitis B virus surface antibody (HBsA...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides an enzyme-catalyzed conductivity immune sensor based on a micro-gap array electrode and a method which is used for detecting infectious virus, and the sensor comprises a micro-gap array electrode, an electrode substrate which is carried out the silanization treatment and enzyme sediments with great conductivity. The invention utilizes the micro-gap electrode array immune sensor to carry out the detection of human immunodeficiency virus (HIV) antibody and hepatitis B virus surface antigen (HBsAg), thus providing the diagnosis method of infectious diseases with rapidity, high sensitivity and high throughput and possibly providing the effective technical means for the early diagnosis and the on-site screening of the infectious diseases.

Description

technical field [0001] The invention belongs to the field of biological analysis, in particular to an immune sensor based on a micro-gap array electrode and a method for detecting infectious viruses by using the immune sensor. Background technique [0002] Infectious diseases are contagious diseases produced by pathogenic microorganisms and parasites infecting the human body. It can spread rapidly among people and cause epidemics, causing great harm to human life and health. How to effectively deal with the outbreak, prevalence and spread of various acute and chronic infectious diseases has become one of the major concerns of governments around the world today. Currently, immunoassays for infectious viruses mainly include enzyme-linked immunosorbent assay (ELISA) and colloidal gold immunochromatography. Although these methods are relatively fast and simple, they can only achieve qualitative or semi-quantitative detection and cannot accurately measure the concentration. Mor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/569G01N33/535G01N27/327
Inventor 俞汝勤蒋健晖沈国励楚霞黄勇
Owner HUNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com