Vaccine for differentiating porcine actinobacillus pleuropneumonia serum 7-type double-gene deletion mutant of vaccine immunity and virus infection animal and application thereof

Abstract

The invention belongs to the animal bacteria genetic engineering field, and relates particularly to construction of a vaccine for a serotype-7 double gene deletion mutation strain for differentiating actinobacillus pleuropneumoniae of swine of a vaccine immunized animal and a wild virus infected animal, and the application of the strain. In the invention, a serotype-7 double gene deletion mutation strain APP-7-mut01 for differentiating actinobacillus pleuropneumoniae of swine of a vaccine immunized animal and a wild virus infected animal (with accession number of CCTCC NO:M207004) is obtained. The strain deletes two main virulence factors of apxIIC and apxIVAN of APP serotype-7. The mutant strain is safe for swines, and has toxins of ApxIIA and ApxIVAC protein, thereby ensuring the immunity efficacy of the vaccine. The vaccine of the mutant strain is used to immunize animals, so that antibodies aiming at Apx IVAN protein are no longer produced inside the animals, however wild virus infection can produce antibodies aiming at ApxIVAN. The invention further discloses the construction, the vaccine preparation and the application of the double gene deletion mutation strain.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering of animal bacteria, and in particular relates to the construction, vaccine preparation and application of an actinobacillus pleuropneumoniae serum type 7 gene deletion mutant strain for distinguishing between vaccine-immunized and wild virus-infected animals. Background technique [0002] Porcine contagious pleuropneumonia (PCP) is a porcine contagious respiratory disease caused by Actinobacillus pleuropneumoniae (APP), which has caused serious economic losses to the world's pig industry. Since the prevalence of PCP was discovered in our country, the vast number of veterinary workers have conducted a series of researches on its etiology, epidemiology, diagnosis and control, and achieved certain achievements. But at present, the incidence of this disease in our country is still increasing year by year, and the positive rate of some pig farms has reached more than 70%, which has become o...

AI Technical Summary

Problems solved by technology

Compared with the three toxins ApxI, ApxII and ApxIII, the discovery and research of the toxin ApxIV started relatively late. Most of the current research on ApxIV is based on the cloning and expression of some ApxIV genes for diagnostic purposes. In-depth research on the role in immunity and vaccines has not been reported at home and abroad

Images