Disease-resistant correlated wheat MYB albumen, coding gene and application thereof

A technology for encoding genes and proteins, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of MYB protein gene cloning, expression characteristics and function research that have not been reported yet

Inactive Publication Date: 2008-09-17
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the cloning, expression characteristics and functional studies of the MYB protein gene related to wheat disease resistance

Method used

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  • Disease-resistant correlated wheat MYB albumen, coding gene and application thereof
  • Disease-resistant correlated wheat MYB albumen, coding gene and application thereof
  • Disease-resistant correlated wheat MYB albumen, coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1. Obtaining of TaPIMP1 and its coding gene TaPIMP1 related to plant disease resistance

[0048] 1. Amplification of the 5'end sequence

[0049] The Gibberella-resistant wheat variety Sumai No. 3 (purchased from the Germplasm Bank of Jiangsu Academy of Agricultural Sciences) that grew to the two-leaf stage was treated with Gibberella, and the specific treatment method was: Fusarium graminearum (Jiangsu Agricultural) (Institute of Biotechnology of the Chinese Academy of Sciences) Mycelium blocks were inoculated by friction inoculation to the leaves of Sumai No. 3 wheat that grew to the two-leaf stage, and the Gibberella hyphae blocks were placed in the leaf sheaths. After 48 hours, the wheat treated with Gibberella hyphae was cut out. The leaves, treated with liquid nitrogen, were operated in accordance with the instructions of the Invitrogen TRIZOL Reagent total RNA extraction reagent manual to extract the total RNA from the above-mentioned wheat leaves. According t...

Embodiment 2

[0060] Example 2. Induced expression analysis of TaPIMP1 gene

[0061] Scab mycelium blocks were used to rub inoculate the leaves of Sumai No. 3 wheat cultivated in flowerpots for 10 days, and the blocks of scab mycelium were placed in the leaf sheaths of wheat; Rhizoctonia cerealis (Jiangsu Academy of Agricultural Sciences) The seedlings of Rhizoctonia solani (National Germplasm Bank of China) resistant to sheath blight (China National Germplasm Bank) seedlings were cultured in flowerpots for 15 days (two-leaf stage) with mycelium blocks by friction inoculation of mycelium blocks, and the blocks of sheath blight mycelium were placed in the leaf sheaths. After 48 hours, the wheat leaves with the above-mentioned different treatments were taken, and stored in an ultra-low temperature refrigerator at -80°C after quick freezing in liquid nitrogen.

[0062] The RNA from the wheat leaves with different treatments was extracted (approximately 5 μg total RNA for each sample), and reverse ...

Embodiment 3

[0064] Example 3 Analysis of disease resistance of transgenic tobacco with TaPIMP1 gene

[0065] 1. Obtainment of transgenic tobacco with TaPIMP1 gene

[0066] Use the plasmid pT-TaPIMP1 containing the full-length ORF of TaPIMP1 gene as template, and MBD-F: 5-CA with XbaI and BmaHI restriction sites TCTAGA ATGGACATGGACAAGG-3 and

[0067] MBD-R: 5-GT GGATCC TTCATCGTCAGCAGTAA-3 is a primer to amplify the ORF of TaPIMP1 by PCR. The 982bp PCR product was digested with XbaI and BmaHI, and then ligated to the large fragment obtained by digesting vector pBI121 (Beijing Baierdi Company) with XbaI and BmaHI. The ligation product was transformed into E. coli TOP10 competent cells. Recombinants were screened from the kanamycin-resistant colonies, and sequenced analysis was performed. After sequencing, the recombinant plasmid containing the ORF of TaPIMP1 gene was named pBI121-TaPIMP1, and the recombinant plasmid was controlled by the 35S promoter. Transform pBI121-TaPIMP1 into Agrobacterium...

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Abstract

The invention discloses wheat MYB protein correlated to plant disease resistance, encoding gene and application thereof. The protein is protein (a) and protein (b): protein (a) is composed of amino acid sequence of sequence 2 in a sequence table; and protein (b) is made by performing substitution and / or deletion and / or addition of one or more amino acid residues on amino acid residue sequence of the sequence 2 in the sequence table, is correlated to flowering time, and is derived from protein (a). The wheat MYB protein correlated to plant disease resistance is transferred into plants to improve disease resistance of the plants. The method for culturing transgenic plant having improved disease resistance has important theoretical and practical significance and exerts important effect on genetic improvement of the plants.

Description

Technical field [0001] The invention relates to a wheat MYB protein related to disease resistance and its coding gene and application. Background technique [0002] With the changes in farming systems, fertility and water conditions, and climate conditions, wheat diseases and insect pests are becoming more and more serious to my country's main wheat production, and airborne bacterial diseases such as wheat powdery mildew and rust have become the main wheat diseases in my country. At present, powdery mildew has spread to more than 20 provinces and cities in my country, and soil-borne diseases such as sheath blight, root rot and total erosion are becoming more and more serious in the main wheat producing areas. In addition to the prevalence of head blight in the middle and lower reaches of the Yangtze River, there are also In the northward trend, viral diseases such as yellow dwarf disease are expanding and rising. In addition, most of the wheat-growing areas in China have been thr...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/11C12N15/82A01H1/00A01H5/00C12N1/21C12N5/10C12N15/63
Inventor 张增艳董娜辛志勇
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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