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An immunoglobulin and porcine PRRS technology is applied in the field of preparation of anti-porcine PRRS immunoglobulin, and achieves the effects of good safety, good curative effect and high titer.
Inactive Publication Date: 2008-08-27
TIANJIN SHENGJI GRP CO LTD
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Problems solved by technology
At present, there is no report about the preparation method of pig PRRS and immunoglobulin
Method used
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Embodiment 1
[0018] A preparation method of anti-pig PRRS immunoglobulin is characterized in that the specific implementation steps are as follows:
[0019] (1) Basic immunization. Choose healthy animals, such as several cattle, and inject 30-50 pig blue ear vaccines into the neck of each recipient animal for the first time, and carry out basic immunity of pig blue ear virus vaccine. Pig blue ear vaccine adopts intramuscular injection, intradermal, subcutaneous multi-point injection, Houhai point injection and other immunization routes. Mix inactivated porcine PRRS virus cultured by tissue and complete Freund's adjuvant in a ratio of 1:1, and then use it as an immunogen to immunize cattle and donkeys, and inject 30-50 portions of each recipient animal intramuscularly at multiple points in the neck for the first time Pig blue ear vaccine for basic immunization.
[0020] (2) Booster immunization is carried out 8-12 days after the basic immunization; 100-200 doses of porcine PRRS vaccine are...
Embodiment 2
[0025] A preparation method of anti-pig PRRS immunoglobulin is characterized in that the specific implementation steps are as follows:
[0026] (1) Preparation of immune plasma: immunize animals with tissue culture inactivated virus, after basic immunization and hyperimmunization, blood collection and separation of plasma.
[0027] (2) Refining and purification: Dilute the prepared immune plasma by 3 times and then concentrate it to a protein content of 3-15% and a titer content of 3000-8000 through conventional preparation processes such as gastric enzyme digestion, heating denaturation, and ultrafiltration. unit / ml. Add hydrochloric acid to adjust to pH 2.0, 4.0°C, stand for 2 days to inactivate the virus, then add NaOH to adjust to pH 7.0 to kill other susceptible viruses of different animals.
[0028] (3) Sub-packaging: The present invention can have two types of liquid and freeze-dried, and the specifications are 400IU / bottle for treatment and 80IU / bottle for prevention;...
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Abstract
The invention relates to a preparation method of anti porcine blue diseasc immunoglobulin, which comprises (1), mixing inactivated porcine blue diseasc virus and freund complete adjuvant cultured via tissue at the mol ratio of 1:1 as immunogen immune bovine and donkey, while each acceptor animal is injected with 30-50 portions of porcine blue diseasc vaccines at multipoints on neck, (2), after 10-15 days of immunity, processing reinforced immunity via 100-200 portions of porcine blue diseasc vaccines, (3), using natural compresson coagulation method to separate immunoglobulin, depositing impure proteins via ammonium sulfate, cutting Fc segment of pepsin, keeping antibody F(ab) second segment, depositing Fc protein via ammonium sulfate, removing Fc protein, adding hydrochloride to adjust pH to 2.0, standing for 2 days at 4.0DEG C, disinfecting, adding NaOH to adjust pH 7.0, killing other animal susceptible viruses, (4) adding antibiotics into blood, standing for 5-10h, separating serum, separating and extracting immunoglobulin via caprylic acid precipitation and ammonium sulfate precipitation, (5) filtering and removing bacterial, packing without germ. The invention has simple preparation and simple application.
Description
technical field [0001] The invention belongs to the field of medicine, in particular to a preparation method of anti-pig PRRS immunoglobulin. Background technique [0002] At present, porcine viral diseases are still the main infectious diseases that seriously restrict the healthy development of the pig industry. Viral infectious diseases occur in hundreds of millions of pigs in different seasons every year in the country. Once the pig is infected, it will have a sudden onset, severe clinical symptoms, easy to spread, and high mortality and mortality, which is extremely harmful to the breeding industry. [0003] According to the results of PCR detection of PRRS antigen in 121 pig farms across the country by relevant authoritative organizations, 91 positive farms were detected, with a positive rate of 75.2%. There are two serotypes of the virus: American and European. However, judging from the current incidence characteristics of PRRS, the incidence has changed from reprod...
Claims
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