Technique for suspension cultivation of algam dendrobium nobile embryoid of medicinal effective composition of native plant strain

Abstract

The invention relates to a technical process flow that an embryoid is directly induced by adopting the strain stem segment dormancy of Dendrobium candidum which is grown in wild field, the one half MS mineral composition by CH1000mg / L (the components of the one half MS. mineral salt are: 2.5 percent sucrose and 0.5 percent maltose which are taken as carbon sources, 5 percent banana juice, B150mg / L, B 650mg / L, NAA and BA appropriate amount of solid culture medium) is subject to continuous screening for 3 to 4 months on a rotary shaking bed at 90 turns / minute, 29 DEG C plus or minus 1 and the natural indoor light condition, so as to obtain the 8.4 to 9.2mg / g.day, the solid-liquid rate achieves 25 to 35 percent, the cultured embryoid cell nucleus DNA is completely the same as the original plant cell nucleus DNA, the polysaccharide content achieves more than 16 percent, the dry yield rate is more than 10 percent, and the technical parameters are stable annually.

Description

Technical field: [0001] The invention belongs to the field of biotechnology. The invention relates to a biotechnology cloning project of higher plants, especially for Dendrobium officinale which grows very slowly in the natural environment, has very harsh requirements on the environmental conditions, and has very low biomass. At present, the natural resources are very exhausted, endangered and precious. of Chinese herbal medicines. technical background: [0002] "The 21st century is the century of biotechnology". With the rapid development of the urbanization process and the continuous growth of population, the continuous degradation of the ecological environment has made many precious Chinese medicinal materials exhausted and endangered. Especially species like Dendrobium officinale, which grow slowly, are very demanding on the environment, and have extremely low biomass, are "bear the brunt". Although since the 1980s, people have attempted to expand the production of De...

AI Technical Summary

Problems solved by technology

Generally speaking, it is difficult to guarantee the "authentic" standard of medicinal materials where genetic variation is likely to occur through the process of dedifferentiation.

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