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Novel method for preparing vine seed extract

A technology of grape seed, extract, applied in the field of medicine

Inactive Publication Date: 2008-08-20
巫军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that this method also has certain defects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] The following examples further describe the separation and preparation of grape seed extract

[0012] Take 100g of grape seeds, pulverize, and extract 3 times with water in an ultrasonic extractor. The amount of water used is 500ml each time. The extracts are combined and concentrated to a certain volume, and then activated carbon is used for adsorption and decolorization, and then the cavitation suspension extraction separation device is used. First extract with 600ml of ethyl acetate, recover the ethyl acetate, extract the aqueous solution with 600ml of chloroform, recover the chloroform, extract the aqueous solution with 600ml of n-butanol, recover the n-butanol to obtain a crude grape seed extract, which is adsorbed through macropores The resin is eluted with water and 60% ethanol in sequence, and the 60% ethanol eluate is collected, and the solvent is recovered to dryness to obtain grape seed extract. The content of polyphenols is more than 95%, and the content of olig...

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PUM

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Abstract

The invention discloses a craft of effectively extracting polyphenolic compounds from grape seeds. The technological process comprises the following steps: weighted grape seeds, after being dried and crushed, are put into an ultrasonic wave extractor for extraction; the extract is concentrated into a certain volume and then decolorized with the help of active carbon; then by utilizing cavitating suspension-extracting and separating device, ethyl acetate is used for extraction and recovered; the chloroform is used to extract aqueous solution and recovered; finally butanol is used to extract the aqueous solution and recovered; and crude extraction from grape seeds is got after the recovery of the butanol; the crude extraction from grape seeds is dissolved in water, eluted successively by water and 60% ethanol through macroporous adsorption resin; 60% ethanol eluent is collected and the solvent is recovered, and thus the extraction from grape seeds is obtained.

Description

Technical field [0001] The invention relates to the technical field of medicine, and is an efficient extraction process for grape seed extracts, specifically extracting an extract containing polyphenolic compounds such as proanthocyanidins from grape seed as a raw material. Background technique [0002] The planting area and output of grapes in my country rank first in the world. In the process of grape juice and wine making, a large number of by-products such as grape seeds and grape skins are produced. At present, most wineries often discard or ferment it and use it as fertilizer. This treatment method not only causes a waste of resources, but also pollutes the environment. With the steady development of the wine industry, the nutritional and health effects of wine, especially the health effects of polyphenols, have set off a research climax all over the world. Studies have found that grape seed polyphenols are much richer in content and variety than grape skin and pulp, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/87A61P39/06
Inventor 巫军
Owner 巫军
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