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Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof

A technology of Paenibacillus polymyxa and wettable powder, which is applied in the fields of application, bactericide, botany equipment and methods, etc., and can solve the problem of whether the spore state or non-spore state of Paenibacillus polymyxa is not involved, and wettable powder is not involved. and other problems, to achieve good control effect, wide range of control and high control efficiency

Active Publication Date: 2012-04-25
SHANGHAI ZEYUAN MARINE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0025] Although the patents and / or applications (ZL02151019.9, WO2004 / 050861, US2006 / 0018883) invented by the present inventor relate to Paenibacillus polymyxa granules, they do not relate to wettable powders nor to polymyxa Whether the Bacillus is in a spore state or a non-spore state, and its main control object is bacterial wilt

Method used

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  • Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof
  • Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof
  • Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Bacillus culture technology in embodiment 1 Paenibacillus polymyxa fermented liquid

[0057] In this example, the spore culture process of Paenibacillus polymyxa was realized by optimizing the medium components and changing the culture conditions.

[0058] method:

[0059] (1) Optimization of culture medium

[0060] In this example, suitable carbon sources are selected from starch, dextrin, glycerol, glucose, sucrose, rice, and the like. Screen the appropriate nitrogen source from silkworm chrysalis powder, soybean powder, peanut cake powder, protein powder, meat extract, rice sugar, wheat bran, yeast powder, corn steep liquor, ammonium salt, etc. Select suitable inorganic salts from sodium chloride, phosphate, ammonium sulfate, manganese sulfate, magnesium sulfate, calcium carbonate and other metal salts.

[0061] The spore rate in the fermentation broth is used as the screening standard, and the live bacteria content of the fermentation broth is considered comprehe...

Embodiment 2

[0090] The selection of embodiment 2 Paenibacillus polymyxa wettable powder carrier

[0091] This embodiment screens suitable carriers for Paenibacillus polymyxa wettable powder from white carbon black, diatomaceous earth, starch, light calcium carbonate, talcum powder, attapulgite, peat soil, bentonite, kaolin and mixtures thereof, to The live bacteria content in the product is used as the screening standard.

[0092] Paenibacillus polymyxa HY96-2 was cultured according to the cultivation process described in Example 1 to obtain Paenibacillus polymyxa fermentation broth. Then, the above-mentioned carrier is gradually added into the Paenibacillus polymyxa fermentation broth, mixed until the carrier is completely wetted but there is no obvious free water, the mixture is dried in an oven and then ground to obtain a fine powder. Due to the different adsorption capacities of different carriers, the weight of the fermentation broth adsorbed by the carrier per unit weight is not th...

Embodiment 3

[0095] The screening of embodiment 3 Paenibacillus polymyxa wettable powder adjuvant

[0096] The adjuvants screened in this example include wetting agents and dispersants.

[0097] This embodiment screens suitable wetting agents from sodium dodecylbenzene sulfonate, sodium lauryl sulfate, sodium butylnaphthalene sulfonate, and alkylnaphthalene sulfonate; from sodium hexametaphosphate, sodium hydroxymethyl Sodium cellulose, sodium polyphosphate, polyvinylpyrrolidone, alkylnaphthalenesulfonate polycondensate, polyethylene, alkylnaphthalenesulfonate polycondensate sodium salt, xanthan gum, β-naphthalenesulfonate polycondensate Sodium salts are selected for suitable dispersants.

[0098] The screening of wetting agent is based on the wetting time of wettable powder as the screening standard. The shorter the time, the better the wetting effect; the screening of dispersant is based on the suspension rate of wettable powder. The higher the suspension rate, the better the effect. t...

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Abstract

The invention relates to a wettable powder for paenibacillus polymyxa and the application, which is characterized in that: the wettable powder can be produced through carrier, addition agent and paenibacillus polymyxa fermentation liquor mxing and centrifugal spray drying. The wettable powder for paenibacillus polymyxa can be used singly or compositely stabilized with other reagent. The wettable powder has the advantages of preventing bacterial and fungal soil borne disease of plants through pouring roots and preventing bacterial and fungal leaf disease through spraying.

Description

technical field [0001] The invention relates to a microbial pesticide wettable powder and its application and preparation method. More specifically, the present invention relates to a Paenibacillus polymyxa wettable powder and a preparation method thereof, and the use of the Paenibacillus polymyxa wettable powder in the prevention and treatment of plant bacterial and fungal soil-borne diseases and leaf diseases. application. Background technique [0002] In recent years, with the deterioration of the environment, crop continuous cropping and monoculture, the occurrence of soil-borne diseases and leaf diseases has become more and more serious, which has seriously affected the development of the entire agriculture. Therefore, it is more and more important to solve soil-borne diseases and leaf diseases. For plant bacterial and fungal soil-borne and leaf diseases (such as bacterial wilt, fusarium wilt, angular spot, leaf spot, etc.), so far there is no good chemical pesticide ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01N25/14A01P3/00
Inventor 李元广魏鸿刚沈国敏李淑兰王伟盛保伟李蜀汪荣津陈智旺李际军
Owner SHANGHAI ZEYUAN MARINE BIOTECH
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