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Mycotoxin purification column and mycotoxin purification method

A mycotoxin and purification column technology, applied in chemical instruments and methods, separation methods, solid adsorbent liquid separation, etc., can solve the problems of unsatisfactory purification effect, limited application range, long processing time, etc., and achieve stable properties and applicable Wide-ranging, low-cost effects

Inactive Publication Date: 2011-06-15
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The purpose of purifying mycotoxins in food and feed extracts is to accurately detect the contamination and residual mycotoxins in food and feed in subsequent procedures. Currently, various purification columns and purification methods used for this purification have a long processing time. The application range is limited, the cost is high, and the purification effect is not ideal.

Method used

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  • Mycotoxin purification column and mycotoxin purification method
  • Mycotoxin purification column and mycotoxin purification method
  • Mycotoxin purification column and mycotoxin purification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Items to be inspected: peanut butter;

[0027] Mycotoxin type: aflatoxin;

[0028] Purification column packing configuration: packing 2.5g, including silica gel (Qingdao Hailang Silica Gel Desiccant Factory) 1.35g, neutral alumina (Zhengzhou Inno Chromatography Experimental Technology Co., Ltd.) 0.9g, filter aid (diatomaceous earth ZX-616, Beijing Langjie Pudao Filtration Technology Co., Ltd.) 0.25g;

[0029] The purification method includes the following steps:

[0030] The first step is to prepare the sample solution, extract the target analyte in peanut butter twice with 20ml of acetonitrile / water (v / v: 10 / 90) mixture, and combine the extracts;

[0031] The second step is to load the sample, take 2ml of the extract and add it to the purification column, and collect the effluent;

[0032] The third step is to elute with methanol as the eluent, flow through the purification column with 4ml of acetonitrile, drain under negative pressure, and collect the effluent;

...

Embodiment 2

[0035] Object to be inspected: paddy;

[0036] Types of mycotoxins: aflatoxins, trichothecenes;

[0037] Purification column packing configuration: packing 2.5g, including 0.9g silica gel, 1.35g neutral alumina, and 0.25g filter aid (Celite545, Merck Chemical Technology (Shanghai) Co., Ltd.);

[0038] The purification method includes the following steps:

[0039] The first step is to prepare the sample solution, extract the analytes in the rice twice with 20ml of acetonitrile / water (v / v: 16 / 84) mixture, and combine the extracts;

[0040] The second step is to load the sample, take 2ml of the extract and add it to the purification column, and collect the effluent;

[0041] The third step is to elute with acetonitrile as the eluent, flow through the purification column with 4ml of acetonitrile, drain under negative pressure, and collect the effluent;

[0042] Analyte recovery: 90-105%, relative standard deviation: less than 5%.

Embodiment 3

[0044] Object to be inspected: corn flour;

[0045] Types of mycotoxin contamination: trichothecenes;

[0046] Purification column packing configuration: packing 2.5g, including silica gel 1.8g, neutral alumina 0.45g, filter aid (Celite-hyflosuper-cell, Merck Chemical Technology (Shanghai) Co., Ltd.) 0.25g;

[0047] The purification method includes the following steps:

[0048] The first step is to prepare the sample solution, extract the analytes in the corn twice with 20ml of acetonitrile / water (v / v: 20 / 80) mixture, and combine the extracts;

[0049] The second step is to load the sample, take 2ml of the extract and add it to the purification column, and collect the effluent;

[0050] The third step is to elute with ethyl acetate as the eluent, flow through the purification column with 4ml of acetonitrile, suck dry under negative pressure, and collect the effluent;

[0051] Analyte recovery: 82-98%, relative standard deviation: less than 5%.

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Abstract

The present invention provides a mycotoxin purifying column filled with composite fillings and its purifying method. The purifying column mainly includes a column, a filling material filled in the column and the a sieve plate set on the two ends of the filling material, the filling material is layered, being a super cell, a neutral alumina and a silica gel in turns up and down, the filling grain diameter is 5-100 mu m. The purifying method of the invention uses adsorption theory of the sorbent filling material, selectively absorbs impurity in flowing extracting solution by utilizing filling material, flushes purifying column by eluent, collects sample effluent liquid and eluent. Impurity absorbs on the column, achiving the effect of removing impurity. The advantages of the mycotoxin purifying column are the lower cost, convenient producing, and changable filling ratio if needed; the purifying method has a quick speed, wide application, convenient operation, better purifying effect.

Description

(1) Technical field [0001] The invention relates to a mycotoxin purification column and a method for purifying mycotoxins in a sample by using the mycotoxin purification column. (2) Background technology [0002] The purpose of purifying mycotoxins in food and feed extracts is to accurately detect the contamination and residual mycotoxins in food and feed in subsequent procedures. Currently, various purification columns and purification methods used for this purification have a long processing time. The application range is limited, the cost is high, and the purification effect is not ideal. (3) Contents of the invention [0003] The purpose of the present invention is to provide a low-cost purification column capable of simultaneously purifying multiple types of mycotoxins with an ideal purification effect, and a method for purifying mycotoxins in samples using the mycotoxin purification column. [0004] The technical scheme adopted in the present invention is: [0005]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/22
Inventor 任一平蔡增轩潘红锋谢文徐小民韩见龙冯靓
Owner ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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