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Method of regulating peach seedling-plant transition stage

A kind of seeding and stage technology, applied in the field of using physiological markers to assist in regulating the stage transition of peach seedling trees, to achieve the effects of eliminating sampling errors, reducing workload, and saving breeding costs

Inactive Publication Date: 2010-06-09
CHANGLI INST OF POMOLOGY HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant patent report on the regulation technology of the transition of seed tree stage

Method used

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  • Method of regulating peach seedling-plant transition stage
  • Method of regulating peach seedling-plant transition stage
  • Method of regulating peach seedling-plant transition stage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Sampling

[0037] ①Changli Fruit Tree Research Institute took samples and monitored two year-old natural seed populations of Okubo and Yanhong in 2003. One sprouted tiller was selected from the root neck of peach seedling saplings in spring, sampled on September 15, and the selected sprouted tiller was cut. , starting from the base of the seedling tree, it rises with the node position, and every 5 nodes is a sampling unit. Take 30 seedling trees and mix them with the sampling units at the same node position. Repeat 1 time to select a total of 60 trees, and put them in the ice box. back;

[0038] ② After washing with tap water, remove the phloem, wrap it with gauze and put it into liquid nitrogen for testing;

[0039] 2) Determination:

[0040] The content of abscisic acid ABA, gibberellin GA, isopentenyl adenine iP, zeatin ZT contained in the endogenous hormone contained in the phloem sampling is determined by high performance liquid chromatography, and the conditi...

Embodiment 2

[0052] 1) Sampling: Sampling on August 20, 2003, the steps are the same as in Example 1;

[0053] 2) Determining the content of abscisic acid ABA, gibberellin GA, isopentenyl adenine iP, and zeatin ZT in endogenous hormones in the sampling, the steps are as follows:

[0054] ① Sampling was dried in a freeze dryer for 26 hours, ground into a fine powder, and passed through a 35-mesh sieve;

[0055] ②Take 0.7 g of freeze-dried sample powder, add 3 ml of 100% acetonitrile at 4°C containing 0.8 mmol of 2,6-di-tert-butyl-p-cresol (BHT), place in an ultrasonic bath at 35°C for 25 minutes, and extract in an ice bath 15 hours, centrifuge at 12,000 rpm for 8 minutes at 4°C;

[0056] ③ Take 2 ml of the supernatant obtained in ②, dry at 35°C, redissolve with 0.8 ml of 0.4 mol / L phosphate buffer solution with pH 8.0, add 0.6 ml of chloroform for extraction 5 times until the organic phase Colorless, adjust the pH of the aqueous phase to 8.0 with 25 μl of 1.5 mol / L NaOH, add 90 mg of inso...

Embodiment 3

[0064] 1) Sampling: Sampling on August 30, 2003, the steps are the same as in Example 1;

[0065] 2) Determining the content of abscisic acid ABA, gibberellin GA, isopentenyl adenine iP, and zeatin ZT in endogenous hormones in the sampling, the steps are as follows:

[0066] ① Sampling was dried by a freeze dryer for 22 hours, ground into a fine powder, and passed through a 38-mesh sieve;

[0067] ②Take 0.4 g of freeze-dried sample powder, add 2.5 ml of 100% acetonitrile at 4°C containing 0.9 mmol of 2,6-di-tert-butyl-p-cresol (BHT), place in an ultrasonic bath for 29 minutes at 28°C, and extract in an ice bath 11 hours, centrifuge at 11,000 rpm for 10 minutes at 4°C;

[0068] ③Take 1.4 ml of the supernatant obtained in ②, dry at 28°C, redissolve with 0.9 ml of 0.4 mol / L phosphate buffer solution with pH 8.0, add 0.5 ml of chloroform for extraction 3 times until the organic phase Colorless, adjust the pH of the aqueous phase to 8.0 with 18 μl of 1.8 mol / L NaOH, add 75 mg of ...

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Abstract

The invention relates to a method for regulating the seedling tree stage transformation of peach trees, which continuously measures and analyzes the contents of ABA, GA, iP and ZT in the endogenous hormones of different node samples, so as to determine the starting and finishing critical points of seedling tree stage transformation. Against the critical points, regulation measures can be used to the seedling trees to inhibit the nutrition growth, lower the inflorescence nodes for earlier blooming and fruiting, thus reducing the breeding cycle. In the very year after the seeding of peach seedling tree, the juvenescent phase is passed, while the stage transformation regulation is carried out in the second year; the peach trees bloom and fruit in the third year, and the initial selection of hybrid is finished in the fifth year, which is one years ahead of the time with the conventional technology and save 16.7 percent of breeding cost.

Description

technical field [0001] The invention belongs to a method for breeding peaches, in particular to a method for using physiological markers to assist in regulating the stage transformation of peach seed trees. Background technique [0002] It takes several years or even more than ten years for fruit tree seedlings to bear fruit, which is a technical bottleneck for improving the efficiency of fruit tree breeding. The individual development process of woody angiosperms such as fruit trees is divided into childhood, adult vegetative growth and reproductive growth. The seedling tree shows childishness, and the developmental stage that does not have the ability to form flowers is called childhood; although the fruiting tree has the potential to form flowers, it cannot form flowers under natural conditions, and can form flowers after receiving some kind of induction, and even if it does not The developmental stage in which this induction can continue to form flowers is called the ad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G17/00A01G7/00G01N30/02G01N33/00
Inventor 张新忠刘国俭常瑞丰李春敏俎文芳王广鹏
Owner CHANGLI INST OF POMOLOGY HEBEI ACADEMY OF AGRI & FORESTRY SCI
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