Method for producing lacitc acid by non-calcium autocycle continuous fermentation salt fermentation

Abstract

The invention provides a non-calcium-salt lactic acid production method using automatic circulating continuous fermentation, which is different from the calcium salt method and comprises steps that: size mixing, liquefaction, saccharification, burden, continuous sterilization, membrane filtration, separation of lactic acid, purification and evaporation; the system process water can be treated and recycled respectively through the steps of continuous fermentation and ultra filtration, electrodialytic coupled purification and separation as well as secondary reverse osmosis. So in the prior traditional calcium salt method, the strain transfer and proliferative fermentation problems as using intermittent single tanks are solved; strain automatic circulating is realized as using the continuous fermentation equipment and technique. The non-calcium-salt lactic acid production method has the advantages of improved equipment capacity factor and fermentation conversion rate, prolonged strain metabolism time, improved conversion rate of the carbon source and the specific production rate of the fermentation process, zero-emission of the environmental pollution in the fermentation process and significantly improved product quality; thereby the non-calcium-salt lactic acid is suitable for commercial production of organic acid fermentation.

Description

technical field [0001] The invention relates to a method for producing lactic acid by automatic circulation and continuous fermentation by a non-calcium salt method, belonging to the field of biotechnology. Background technique [0002] The traditional fermentation process adopts the organic acid calcium salt method, which has problems such as long production process, low degree of automation, serious pollution, high cost, and poor product quality. [0003] The process route of the traditional calcium salt method fermentation to produce organic acids is: corn, starch pulping → liquefaction → fermentation → plate and frame filtration → purification treatment → evaporation → acid hydrolysis reaction → decolorization → mash separation → decolorization → fine filtration → concentration → Ion exchange→activated carbon column decolorization→membrane filtration→concentration→finished product packaging. [0004] This process is used in the fermentation process and the batch single-...

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Problems solved by technology

[0004] This process is used in the fermentation process and the batch single-tank fermentation after the strain is transferred and multiplied has low fermentation automation control, easy to infect bacteria, degradation, and variation, resulting in large fluctuations in the quality of the fermentation broth, low conversion rate, and low utilization of equipment, resulting in fermentation equipment. Large one-time investment

And because of the use of CaCO in the fermentation process 3 As a neutralizer to convert organic salts, secondary purification treatment and conversion to organic acids by sulfuric acid acid hydrolysis are required in the production system, resulting in long process flow, serious equipment corrosion, poor purification and separation effect, large system loss, and high yield. Low efficiency, high cost, serious environmental pollution, high labor intensity of workers, poor product quality and other shortcomings

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