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Human embryo kidney 293 cell amplifying protein-free medium

A protein-free culture medium, human embryonic kidney technology, applied in embryonic cells, tissue culture, animal cells, etc., can solve the problems of large differences between product batches, unclear ingredients, and bacteria.

Inactive Publication Date: 2008-07-16
天津百若克医药生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the current medium for culturing human embryonic kidney 293 cells are: at present, animal serum is widely used as a key component of mammalian cell culture medium, mainly bovine serum
However, the disadvantages of animal serum are: expensive, unclear ingredients, large differences between product batches, containing cell growth inhibitory components, and more seriously, may contain bacteria, viruses, etc., resulting in potential biological contamination

Method used

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  • Human embryo kidney 293 cell amplifying protein-free medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0221] The medium consists of basic medium, phosphatidylcholine and trace elements, wherein the composition and ingredients of the basic medium are as follows:

[0222] Composition Mg / L

[0223] Glycine 65

[0224] L-alanine 20

[0225] L-Arginine 300

[0226] L-asparagine monohydrate 15

[0227] L-Aspartic Acid 20

[0228] L-cysteine ​​hydrochloride monohydrate 50

[0229] L-Cystine Dihydrochloride 50

[0230] L-glutamic acid 40

[0231] L-Glutamine 1000

[0232] L-histidine hydrochloride monohydrate 15

[0233] L-Hydroxyproline 20

[0234] L-Isoleucine 52

[0235] L-leucine 72.5

[0236] L-Lysine 15

[0237] L-methionine 32

[0238] L-phenylalanine 11.5

[0239] L-Proline 30

[0240] L-serine 66

[0241] L-threonine 40

[0242] L-Tryptophan 42

[0243] L-tyrosine sodium salt 95

[0244] L-valine 16

[0245] L-ornithine hydrochloride 104

[0246] L-citrulline 94

[0247] D-Biotin 0.01

[0248] Choline chloride 0.5

[0249] D-pantothenic acid 0.01

[025...

Embodiment 2

[0325] The medium consists of basic medium, phosphatidylcholine and trace elements, wherein the composition and ingredients of the basic medium are as follows:

[0326] Composition Mg / L

[0327] Glycine 30

[0328] L-alanine 50

[0329] L-Arginine 100

[0330] L-Asparagine Monohydrate 60

[0331] L-Aspartic Acid 5

[0332] L-cysteine ​​hydrochloride monohydrate 120

[0333] L-cystine dihydrochloride 10

[0334] L-Glutamic Acid 100

[0335] L-Glutamine 200

[0336] L-histidine hydrochloride monohydrate 50

[0337] L-Hydroxyproline 5

[0338] L-Isoleucine 150

[0339] L-Leucine 30

[0340] L-Lysine 50

[0341] L-methionine 10

[0342] L-Phenylalanine 50

[0343] L-proline 5

[0344] L-Serine 300

[0345] L-threonine 10

[0346] L-Tryptophan 150

[0347] L-tyrosine sodium salt 50

[0348] L-Valine 50

[0349] L-Ornithine Hydrochloride 50

[0350] L-Citrulline 350

[0351] D-Biotin 0.001

[0352] Choline Chloride 3

[0353] D-pantothenic acid 0.001

[0354] ...

Embodiment 3

[0429] The medium consists of basic medium, phosphatidylcholine and trace elements, wherein the composition and ingredients of the basic medium are as follows:

[0430] Composition Mg / L

[0431] Glycine 120

[0432] L-alanine 5

[0433] L-Arginine 800

[0434] L-asparagine monohydrate 3

[0435] L-Aspartic Acid 80

[0436] L-cysteine ​​hydrochloride monohydrate 10

[0437] L-cystine dihydrochloride 120

[0438] L-glutamic acid 10

[0439] L-Glutamine 3000

[0440] L-histidine hydrochloride monohydrate 3

[0441] L-Hydroxyproline 50

[0442] L-Isoleucine 30

[0443] L-Leucine 300

[0444] L-lysine 5

[0445] L-Methionine 100

[0446] L-phenylalanine 2

[0447] L-Proline 100

[0448] L-serine 10

[0449] L-threonine 150

[0450] L-Tryptophan 10

[0451] L-Tyrosine Sodium Salt 500

[0452] L-valine 5

[0453] L-Ornithine Hydrochloride 400

[0454] L-citrulline 40

[0455] D-Biotin 0.8

[0456] Choline chloride 0.1

[0457] D-pantothenic acid 0.8

[0458] ...

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Abstract

The invention relates to a culture medium and the usage thereof, which particularly relates to a human embryonic kidney 293 cell amplification culture medium and the usage thereof. The invention pertains to the field of biological products. The human embryonic kidney 293 cell amplification protein-free culture medium includes a basic culture medium and further includes a phosphatidylcholine and trace elements. The human embryonic kidney 293 cell protein-free culture medium does not contain any animal serum or protein, and consists of amino acids, vitamins, inorganic salts, sugars, phosphatidyl choline and trace elements. The human embryonic kidney 293 cell serum-free culture medium can directly replace the conventional culture medium which contains serum, and the human embryonic kidney 293 cell does not need an adaptation process.

Description

Technical field [0001] The invention relates to a culture medium, and more specifically to a human embryonic kidney 293 cell expansion culture medium. Belongs to the field of biological products. Background technique [0002] Cell culture media is a commonly used substance in the field of biological products and is essential in bacterial and cell culture. Human embryonic kidney 293 cells are human kidney epithelial cell lines transfected with adenovirus E1A gene. The shortcomings of the current culture medium for culturing human embryonic kidney 293 cells are: currently, animal serum is widely used as a key component of mammalian cell culture medium, with bovine serum being the mainstay. However, the disadvantages of animal serum are: it is expensive, its ingredients are unclear, there are large differences between product batches, it contains cell growth-inhibiting components, and more seriously, it may contain bacteria, viruses, etc., leading to potential biological cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N5/073
Inventor 黄宗堂唐贺文
Owner 天津百若克医药生物技术有限责任公司
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