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Vaccine containing composite adjuvant and preparation method thereof

An adjuvant and vaccine technology, applied in the biological field, can solve the problems of poor vaccine immune effect, weak immune response, and inability to effectively protect immunized animals, and achieve the effect of improving the intensity of immune response and improving response ability

Active Publication Date: 2011-04-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a more effective immune adjuvant-processed vaccine and a preparation method of the vaccine in view of the weak immune response induced by some existing oil adjuvant vaccines injected into animals and unable to effectively protect the immunized animals
[0004] In order to overcome the shortcomings of poor immune effects of some oil adjuvant vaccines, the present invention provides a new vaccine formula, the vaccine prepared by using this formula can greatly improve the immune response intensity induced by the vaccine, and enhance the ability of immune animals to resist infectious diseases

Method used

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  • Vaccine containing composite adjuvant and preparation method thereof
  • Vaccine containing composite adjuvant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0015] Example 1: The combination of ginsenoside and white oil enhances immunity of foot-and-mouth disease (FMD) vaccine.

[0016] 1. Materials and methods

[0017] 1. Experimental animals: 24 female ICR mice, purchased from Shanghai Experimental Animal Center.

[0018] 2. Antigen and adjuvant: the antigen is inactivated O-type foot-and-mouth disease virus (FMDV), provided by Lanzhou Veterinary Research Institute (LVRI), Chinese Academy of Agricultural Sciences. White oil was purchased from Hangzhou Chemical Materials Co., Ltd. Ginsenosides are products of Hongjiu Company.

[0019] 3. Vaccine preparation: first dissolve ginsenoside with a small amount of physiological saline, then mix with inactivated O-type foot-and-mouth disease virus (FMDV) antigen solution, and then fully mix with oil adjuvant to obtain the product.

[0020] 4. Immunization method: 24 mice were randomly divided into 4 groups, 6 mice in each group. Each mouse was injected intramuscularly with 0.2ml of t...

example 2

[0034] Example 2: Test of immunizing piglets with oil adjuvant foot-and-mouth disease vaccine (O type) containing ginsenoside (500 micrograms / ml)

[0035] 1. Materials and methods

[0036] 1. Experimental animals: 20 40-day-old piglets.

[0037] 2. Vaccine and adjuvant: Type O foot-and-mouth disease vaccine provided by Lanzhou Veterinary Research Institute (LVRI), Chinese Academy of Agricultural Sciences. Ginsenosides (GS) were provided by Hongjiu Company.

[0038] 3. Vaccine preparation: first dissolve ginsenosides with a small amount of normal saline, then add the ginsenoside solution to the vaccine and mix thoroughly so that the content of ginsenosides in each milliliter of vaccine is 0 (as a control) and 500 micrograms respectively.

[0039] 4. Immunization method: 20 piglets were randomly divided into 2 groups, 10 piglets in each group. Each pig was intramuscularly injected with 2ml of the vaccine once, blood was collected before and after the immunization, the serum w...

example 3

[0043] Example 3: Test of immunizing piglets with oil adjuvant foot-and-mouth disease vaccine (O type) containing ginsenoside (1000 micrograms / ml)

[0044] 1. Materials and methods

[0045] 1. Experimental animals: 22 40-day-old piglets.

[0046] 2. Vaccine and adjuvant: Type O foot-and-mouth disease vaccine provided by Lanzhou Veterinary Research Institute (LVRI), Chinese Academy of Agricultural Sciences. Ginsenosides (GS) were provided by Hongjiu Company.

[0047] 3. Vaccine preparation: first dissolve ginsenosides with a small amount of normal saline, then add the ginsenoside solution to the vaccine and mix thoroughly so that the content of ginsenosides in each milliliter of vaccine is 0 (as a control) and 1000 micrograms respectively.

[0048]4. Immunization method: 22 piglets were randomly divided into 2 groups, 11 piglets in each group. Each pig was intramuscularly injected with 2ml of the vaccine once, blood was collected before and after the immunization, the serum ...

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Abstract

The invention discloses a vaccine containing compound adjuvant and a preparation technology thereof, which pertains to the filed of biotechnology and relates to the prevention of animal diseases. The vaccine contains the compound adjuvant which is combined by oil adjuvant and ginsenosides adjuvant, and the content of ginsenosides adjuvant per milliliter vaccine is 20 to 1000 micrograms, and the oil adjuvant accounts for one half of the total volume of the vaccine. The preparation method of the vaccine containing compound adjuvant has the following steps: the ginsenosides is dissolved in a small amount of physiological saline; the ginsenosides liquid is mixed with antigen, and then the mixture is fully emulsified and mixed with the oil adjuvant under the function of emulsifier; or the oil adjuvant and the ginsenosides solution are firstly emulsified and mixed, and then the mixture is mixed with the antigen for emulsion; or the ginsenosides solution is added in conventional adjuvant vaccine and then is emulsified and mixed. The compound adjuvant vaccine of the invention is proved that the invention can induce the higher intensity of immune response compared with the usage of a single adjuvant by the antigen animal immune experiments which take the inactivated food and mouth disease virus as the module.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the prevention technology of animal diseases, in particular to a vaccine using a compound adjuvant and a preparation method thereof. The vaccine prepared by using the compound adjuvant can greatly improve the specific immune response intensity induced by the vaccine. Background technique [0002] Infectious diseases are a class of important diseases that endanger animal health and cause serious economic losses to animal husbandry. Vaccination of animals is a routine method in many countries to prevent the occurrence of infectious diseases. Adjuvants can improve the immune effect of vaccines, and oil adjuvants are an important component of many animal infectious disease vaccines (such as foot-and-mouth disease vaccines). However, the immune effect of some commercial oil adjuvant vaccines is not very satisfactory. For example, the investigation by Xie Qin et al. showed that only 20.9% o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61P31/00
Inventor 胡松华
Owner ZHEJIANG UNIV
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