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Extraction and purification method of 7 Alpha, 15Alpha-dihydroxy androstene alcohol ketone

A technology of hydroxyandrostenolone and a purification method, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, organic chemistry, etc., can solve problems such as inability to extract products, improve extraction efficiency, reduce production costs, and increase total production costs. Yield effect

Inactive Publication Date: 2010-08-11
SHANGHAI INST OF PHARMA IND CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This shows that the existing extraction method can not effectively extract the product

Method used

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  • Extraction and purification method of 7 Alpha, 15Alpha-dihydroxy androstene alcohol ketone
  • Extraction and purification method of 7 Alpha, 15Alpha-dihydroxy androstene alcohol ketone
  • Extraction and purification method of 7 Alpha, 15Alpha-dihydroxy androstene alcohol ketone

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Abstract

The invention provides a method for extracting and purifying 7 Alpha, 15 Alpha-dihydroxyandrostanolone; the 7 Alpha, 15 Alpha-dihydroxyandrostanolone is converted from Colletotrichum lini AS 3.4486 and after the conversion, the supernatant liquid of the centrifugal fermentation liquid is extracted with non-polar organic solution; the invention is characterized in that the method comprises the fungus obtained after centrifugation is extracted with hydrophilic organic solution and then the non-polar solution is used for extraction. By adopting the method for extraction and purification, the yield of the product can reach nearly 90 percent and the total yield of preparing I with the Colletotrichum lini AS 3.4486 can reach 75 percent; in this way, the total yield of converting the Colletotrichum lini AS 3.4486 into I is effectively increased and the production cost of I is reduced.

Description

technical field The present invention relates to a method for extracting and purifying a biotransformation product from a fermentation broth, in particular to a method for effectively converting a substrate dehydroepiandrosterone into a product 7α, 15α-dihydroxyandrostenolone from a fermentation broth Methods of extracting and purifying the product. Background technique Steroid intermediates are often used in the production of various clinical drugs. 7α, 15α-dihydroxyandrostenolone and its analogs (its structure is as follows: Formula I) It is an important steroid intermediate, which can be used in the synthesis of various drugs such as lactone aldosterone receptor antagonists, diuretics and gynecological drugs. For example, I is an important intermediate in the synthesis of drospirenone, the active ingredient of the oral contraceptive drug Yasmin. Microbial transformation plays an important role in the synthesis of steroid drugs. Several important steroid microbial tra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/06C07J1/00C12R1/645
Inventor 胡海峰陶荣盛张琴
Owner SHANGHAI INST OF PHARMA IND CO LTD
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