Culture medium composition adapting for blueberry test tube plantlet radication as well as method thereof

Abstract

The invention relates to a culture medium composition suitable for the rooting of blue berry plantlets and the method. The procedures of the invention are that: first, the preparation of rooting medium and the weight of the rooting medium contained in every liter is that: firstly, a basic medium: 1 / 2WPM culture medium, wherein white sugar 20g / L, pH5.2 to 5.4; secondly, culture medium supporting substance: perlite, 150 to 200g / L; thirdly, the rooting medium: 1 / 2WPM+IBA 0.03 to 0.1mg / L. Second, the single plant cultivated for 40 to 50 days in a multiplication medium and having a length of more than 3 to 5 centimeters is inoculated to the rooting medium; after 20 to 25 days, a plurality of roots are grown at the basal of the seedling. Third, the domestication and the transplantation of tissue culture seedlings: the rooted tissue culture seedlings of the second step having a length of more than 3 to 7 centimeters are directly transplanted into a growing media without washing the root section. The invention has the advantages that: the provided blue berry rooting optimized medium has strong pertinence and good applicability; the culture medium supporting substance can be reused; the transplanting survival rate is more than 90%; running water and white sugar are used in the invention to replace distilled water and sucrose, therefore tissue culture cost is saved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and mainly relates to a medium composition suitable for rooting blueberry test-tube seedlings and a method thereof. Background technique [0002] Blueberry is one of the most promising fruit tree species worldwide in this century. Its fruit is rich in various vitamins, arbutin, anthocyanin, SOD, tannic acid and flavonoids and other antioxidant ingredients and has unique health care functions. Among more than 40 kinds of fruits and vegetables, blue berries have the highest antioxidant activity, which has the effects of eliminating eye fatigue, improving vision, eliminating inflammation in the body, and dilating blood vessels; Obvious curative effect, play a role in delaying the aging of brain nerves, enhancing memory, anti-cancer and so on. Blue berry has excellent processing performance. Its fruit is soft and juicy, with unique fragrance, bright color and st...

AI Technical Summary

Problems solved by technology

But the degree of difficulty that different plants utilize the plant tissue culture method to breed seedlings is different, exist blueberry rooting rate low, rooting period long, rooting program complexity in the document of existing report (first cultivate in dark 20 days and then cultivate in light for 20 days) days, and the rooting rate is only 30%-70%) and the low survival rate of transplanted seedlings, making it impossible to realize large-scale factory production