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Standard reagent kit for mass spectrometry clinical diagnosis and the method

A technology for standard materials and diagnostic reagents, which is applied in the field of protein detection and can solve the problem of lack of clinical mass spectrometry diagnostic reagents.

Inactive Publication Date: 2008-04-23
许洋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, we found that there is no standard substance kit for clinical mass spectrometry diagnostic reagents when performing protein mass spectrometry analysis

Method used

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  • Standard reagent kit for mass spectrometry clinical diagnosis and the method
  • Standard reagent kit for mass spectrometry clinical diagnosis and the method
  • Standard reagent kit for mass spectrometry clinical diagnosis and the method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Standardized quality control serum (plasma) preparation of embodiment 1 mass spectrometry

[0055] (1) Experimental method

[0056] 1. Materials

[0057] Specimen source: Standardized quality control serum (plasma) preparation for mass spectrometry. The definitions meet the following criteria. The blood donors are half male and half male, and the blood type is O; the age is 18-30 years old; ethnicity, Han. The biochemical indicators were normal, including: total cholesterol, triglycerides, fasting blood sugar, hepatitis B surface antigen, liver function test, kidney function test; no family history of genetic diseases; no history of major infectious diseases. Women without pregnancy, men with no history of smoking. Fresh blood was drawn from 10 healthy subjects with blood type O (half male and half female), and centrifuged at 4°C immediately after the blood coagulated, at 10,000rpm, 4°C for 5min; sample processing and storage. Take 10 μl of mixed serum (slurry), dilu...

Embodiment 2

[0062] Example 2 Analysis of serum (plasma) proteome has good reproducibility

[0063] The above-mentioned mixed serum (serum) was spotted on 8 spots on the same chip for repeatability detection, and the peptides and low molecular weight proteomes in the serum (serum) were analyzed. like figure 1 As shown, among the 79 main peaks defined and analyzed, the coefficients of variation (CV) of the peak intensities are all below 10%, indicating that the analysis of the proteome by this method has good repeatability.

Embodiment 3

[0064] Example 3 Effects of different storage conditions of serum (slurry) on the results of the proteome

[0065] We put the serum (serum) at 4°C for different times: 0, 2, 4, 6, 8, 12, 24, and 72 hours, and found that within 2 hours, the proteome of the serum (serum) basically did not change, and the serum (serum) None of the 79 peaks defined in Slurry had a CV > 30%. , only 2 peaks (2.5%) had CV>20%. By 4 hours, there were 2 peaks (2.5%) CV>30% and 5 peaks (6.4%) CV>20%; after 12 hours, there were 5 peaks (6.4%) CV>30% and 10 peaks (12.7%) had CV > 20%. Time extended to 72 hours, there were 10 peaks (accounting for 12.7%) CV>30% and 19 peaks (accounting for 24.1%) CV>20% ( figure 2 ).

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Abstract

The standard substance kit is a standardization quality-controlled blood serum sampler packaged in tubule. Under -80 deg. C, the pooled serum is taken out and is diluted in U9 buffer solution in 1:2 mixing ratio; saving it at 25 deg. C. A quantification mass spectrographic analysis under the control of standardization quality-controlled O type blood serum is used for test.

Description

technical field [0001] The invention relates to a new standard substance and kit for the protein analysis method in biological samples. One captures biomarkers through a protein-binding matrix and detects them using quantitatively controlled mass spectrometry. The invention mentioned here relates to the field of protein detection and is a new non-invasive in vitro mass spectrometry detection method. The present invention can be applied to the standard substance detection method or the standard substance kit development of the biomarker combination in the body fluid that has been separated from the human body. Background technique [0002] With the implementation and completion of the Human Genome Project, scientists proposed the concept of the post-genome (post-genome) project, and the focus of research shifted to functional genomics, and the main substance of biological function is protein. In 1994, Wilkins and Williams of Macquarie University in Australia first proposed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N33/48
Inventor 许洋
Owner 许洋
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