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Super-paramagnetic composite particle drug-loaded body and preparation method thereof

A composite particle and superparamagnetic technology, which is applied in the direction of pharmaceutical formulations, antineoplastic drugs, drug combinations, etc., can solve problems not related to drug-carrying bodies and preparation methods, achieve flexible drug-loading methods, improve curative effect, and drug packaging The effect of high coverage

Active Publication Date: 2008-04-23
XIAN GOLDMAG NANOBIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the published patent documents only involve the labeling of nucleic acids, oligonucleotide probes, antigens, antibodies, enzymes, polypeptides, polysaccharides, avidin, streptavidin or cells on the surface of gold magnetic particles and the application of gold magnetic particles in biological and Applications in non-biological assays, not related to drug carriers and preparation methods

Method used

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  • Super-paramagnetic composite particle drug-loaded body and preparation method thereof
  • Super-paramagnetic composite particle drug-loaded body and preparation method thereof
  • Super-paramagnetic composite particle drug-loaded body and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The drug is directly coated by using the gold shell layer of gold-magnetic composite particles or the high surface activity of colloidal gold to form drug-loaded particles. The drug in this example is doxorubicin.

[0050] Shake the 5mg / ml gold magnetic composite microparticle suspension, pipette 200μl into a centrifuge tube, add 400μl of pH 7.4 phosphate buffer, shake gently to resuspend the magnetic particles, and then place the centrifuge tube in Magnetically separate in a magnetic separator for 2 minutes, pipette the supernatant on the magnetic separator and discard; then add 200-400 μl of 0.5 mg / ml doxorubicin normal saline solution, blow and beat to mix, and place in a shaker Bed, at 25°C, oscillating at 200r / min for 5-6 hours; after completion, magnetically separate, remove the supernatant and discard, to obtain doxorubicin-loaded gold-magnetic composite particles. Store at 4°C. Before use, add 1-2ml of normal saline to dilute. Note that it should be used as soo...

Embodiment 2

[0052] The surface of the gold-magnetic composite microparticles is sequentially coated with polymer materials and drugs to form drug-loaded microparticles. The polymer material in this example is bovine serum albumin (BSA), and the drug is doxorubicin.

[0053] After shaking the 5mg / ml gold magnetic particles, use a pipette to pipette 200μl into a centrifuge tube for magnetic separation, add 400μl of pH 7.4 phosphate buffer, shake fully, and place the centrifuge tube on a magnetic separator. After separation for 2 min, the supernatant was removed with a pipette on a magnetic separator and discarded. Add 200-400 μl of 2 mg / ml BSA phosphate buffer solution, place on a constant temperature shaker, shake at 37°C and 200 r / min for 1-2 hours. After coating, magnetically separate, remove supernatant and discard, wash twice with 50% ethanol, then disperse in 0.8-1ml doxorubicin solution of 0.5mg / ml, shake at room temperature for 4-6 hours. After completion, magnetic separation was ...

Embodiment 3

[0055] The principle of preparation is the same as in Example 2, wherein the polymer material is chitosan, and the drug is doxorubicin.

[0056] Weigh 1g of chitosan and place it in a 250ml three-neck flask, add 10ml of 3% acetic acid solution, stir to make it fully dissolved, then add 5-10ml of 5mg / ml gold magnetic particles, shake well and ultrasonically for 10min (room temperature, 80mHz ), placed in a water bath at 40°C, and stirred at 300-800r / min for 1-2 hours. Magnetic separation, washed several times with ultrapure water. Then ultrasonically suspend in 0.8-1ml adriamycin solution of 0.5 mg / m1, and shake for 4-6 hours at room temperature. After the completion, magnetic separation is performed, and the supernatant is removed and discarded to obtain doxorubicin-loaded chitosan gold-magnetic composite particles. Store at 4°C. Before use, add 1-2ml of normal saline to dilute.

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Abstract

The present invention relates to a superparamagnetic composite microparticle medicine-carrying body which can be mainly used for making magnetic target therapy and its preparation method. The invented magnetic composite microparticle medicine-carrying body is composed of magnetic composite microparticle and medicine-carrying layer which can be used for covering exterior of said magnetic composite microparticle. Said invention is characterized by that it utilizes the property of high surface activity of gold shell layer or colloidal gold of superparamagnetic composite microparticle to directly cover medicine or firstly utilizes the property of high surface activity of the gold shell layer or colloidal gold of superparamagnetic composite microparticle to make its surface be covered with high-molecular material, then utilizes the affinity adsorption or covalent linkage of high-molecular material to medicine to cover the medicine, also can make the surface of superparamagnetic composite microparticle be covered with the high-molecular material and medicine cross-linked or target preparation and medicine cross-linked or high-molecular material, target preparation and medicine cross-linked composite body layer.

Description

technical field [0001] The invention relates to a technique for compounding drugs with superparamagnetic composite microparticles as a carrier, in particular to a superparamagnetic composite microparticle drug-carrying body mainly used for magnetic targeting therapy and a preparation method thereof. Background technique [0002] With the development of nanotechnology, people have begun some research on the application of nanoparticles as drug carriers, especially in targeted therapy. [0003] In the 1970s, Widder et al. first proposed the targeted therapy of magnetic drugs, and proposed the concept of loading chemotherapy drugs on biocompatible magnetic particles for in vivo treatment. 3 o 4 Doxorubicin was immobilized on magnetic particles and applied to the test of treating tail tumors in rats (Widder K J et al, Magnetic microsphere: A model system for sitespecific drug delivery in vivo[J], Prc Soc Exp Biol Med, 1978, 158( 1): 141-146). The magnetic albumin microspheres...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61K47/36A61K47/34A61K47/32A61K47/02A61K9/16A61K45/00A61K48/00A61K31/704A61P35/00A61K47/10
Inventor 陈超崔亚丽张腾霄张华
Owner XIAN GOLDMAG NANOBIOTECH
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