Promoter for introducing extracellular substance into mammalian ovum and introduction method

Abstract

It is intended to provide a method, upon introducing an extracellular substance such as a sperm, a cell nucleus, a nucleic acid or a protein into a mammalian ovum, for introducing the extracellular substance into the mammalian ovum, which is safe for the mammalian ovum, enables efficient introduction, and is technically easy. By treating the mammalian ovum with a tubulin polymerization inhibitor such as vinblastine, membrane fusion between the ovum and the sperm is promoted and without making a hole in the cell membrane, the sperm and the ovum are fused and fertilization can be achieved. The method of the invention can be applied not only to the case where a sperm is introduced into a mammalian ovum, but also to a case where an extracellular substance such as a cell nucleus, a nucleic acid or a protein is introduced into a mammalian ovum.

Description

technical field [0001] The present invention relates to an extracellular substance introduction promoter for mammalian eggs containing a tubulin polymerization inhibitor as an active ingredient, and a method for introducing extracellular substances into mammalian eggs using the introduction promoter. More specifically, using Extracellular substance introduction promoter for mammalian eggs containing tubulin polymerization inhibitor as an active ingredient, method for introducing extracellular substances such as sperm, nucleus, nucleic acid, and protein into mammalian eggs, and breastfeeding for the method Animal egg import promoter for extracellular substances. One of the characteristics of the present invention is to provide an attempt to improve the fertilization ability of mammalian eggs by treating mammalian eggs with an extracellular substance introduction promoter containing a tubulin polymerization inhibitor as an active ingredient, and to promote the transfer of sperm ...

AI Technical Summary

Problems solved by technology

In other words, the problems of the ICSI method are considered to be: (1) technical cultivation is required when the ICSI method is used to introduce extracellular substances into mammalian eggs, and (2) the ICSI method is used to introduce extracellular substances into mammalian eggs. The material method has a low success rate. (3) The ICSI method is performed by opening a hole in the egg cell membrane with a glass capillary, so opening the hole will cause adverse effects on embryogenesis, etc.

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