Unfreezing liquid for frozen semen of equine animal

A technology of freezing semen and thawing liquid, which is applied in the fields of animal delivery, human or animal body preservation, medical science, etc. It can solve the problems of low conception rate and poor recovery of sperm motility, so as to ensure the survival rate and enhance the fertilization ability, The effect of improving the utilization rate

Inactive Publication Date: 2014-04-30
QINGDAO DERUI JUNFA BIOLOGY TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems that the traditional semen thawing liquid has poor recovery of sperm motility, low conception rate and ready-to-use and ready-to-use problems, the invention provides a frozen semen thawing liquid for equine animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Weigh glucose 24g, raffinose 1.6g, D-lactose monohydrate 1.6g, trisodium citrate Na 3 C 6 h 5 o 7 2H 2 Put O 0.24g, potassium citrate 0.4g and HEPES 4.7g into a sterile beaker, add double distilled water to dissolve, constant volume, filter, sub-package, sterilize and seal to obtain liquid A; Bacteria and sealed liquid A marked the preparation time, stored at 0-5°C, and taken as needed; take an appropriate amount of liquid A and add an equal volume of skim milk to mix evenly to obtain the thawed liquid of Example 1.

[0014] Comparative test 1

[0015] The traditional thawing solution glucose-yolk dilution (control group 1), milk thawing solution (control group 2), and the thawing solution of Example 1 (example group 1) were used to thaw the frozen semen of horses respectively. After thawing, observe the sperm motility of control group 1, control group 2 and embodiment group 1 within 0-10 hours, and measure the motility rate of sperm every 2 hours. The results are ...

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PUM

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Abstract

The invention discloses an unfreezing liquid for frozen semen of an equine animal. The unfreezing liquid includes a solution A and skimmed milk; and the solution A includes glucose, melitose, D-lactose monohydrate, trisodium citrate, potassium citrate and HEPES. A preparation method of the unfreezing liquid is as below: weighing ingredients in the solution A and adding the ingredients in a sterile beaker; adding double distilled water for dissolving; reaching a constant volume, filtering, filling, sterilizing and sealing to obtain the solution A; and adding an equivalent amount of skimmed milk to the solution A, and mixing evenly to obtain the unfreezing liquid. The unfreezing liquid is suitable for unfreezing of frozen semen of equine animals. The invention has the beneficial effects of simple preparation, convenience for use, good unfreezing effect of frozen semen of the equine animal, multiple applications of the unfreezing liquid, time saving and labor saving, and is suitable for large-scale production and application.

Description

technical field [0001] The invention relates to the technical field of animal semen preservation, in particular to a frozen semen thawing solution of equine animals. Background technique [0002] The combination of semen cryopreservation and artificial insemination technology plays an extremely important role in the production of modern breeding industry. The application of frozen semen for artificial insemination can not be limited by time, region, variety and individual physique, and can greatly reduce the number of male animals and reduce the The production cost is reduced, the utilization rate of high-quality breeding males is improved, and huge economic benefits are brought to the breeding industry. At the same time, the development of semen freezing technology and the use of frozen semen are of great significance in the introduction of varieties, hybrid improvement, improvement of reproductive performance, disease control and protection of genetic diversity. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02A61D19/02
Inventor 邹志钢张肖军马玉萍
Owner QINGDAO DERUI JUNFA BIOLOGY TECH
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