Method for vitrified freezing and thawing of canine ova, and ova frozen and thawed according to same

A vitrification and vitrification technology, applied in the field of vitrification and thawing of canine eggs, freezing and thawing eggs, can solve the problems of less than 5% production efficiency, results and technical shortages, etc., and achieve high nuclear transfer and fertilization. , the effect of reducing toxicity, high nuclear transfer culture effect

Pending Publication Date: 2020-12-22
艾牧克生物技术有限责任公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the production efficiency of somatic cell cloned animals is known to be less than 5% (Heyman et al., 2002; Yang et al. 2008)
Therefore, when the experimental dogs in estrus at the same time cannot be ensured, the technique of securing a sufficient number of eggs by vitrification is required, but at present, there is a shortage of such experimental results and techniques

Method used

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  • Method for vitrified freezing and thawing of canine ova, and ova frozen and thawed according to same
  • Method for vitrified freezing and thawing of canine ova, and ova frozen and thawed according to same
  • Method for vitrified freezing and thawing of canine ova, and ova frozen and thawed according to same

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Embodiment Construction

[0051] The present invention relates to a method for vitrifying and thawing ovum of a canine animal, performing the process described below: a process of collecting mature ovum of a canine animal (1 process), performing a process of vitrifying the above-mentioned collected ovum (2 process) ; Including: the process of preparing mixed solution, equilibrium solution, and vitrification solution (2 process-1); the process of removing the cumulus cells of the recovered ovum (denuding) (2 process-2); The process of treating the mature ovum without cumulus cells with the mixed solution (2 process-3); adding the balance solution to the mixed solution containing the ovum and treating it, and then adding the balance solution treatment process (2 process-4) ; The process of treating the treated ovum with the balance solution again (2 process-5); the process of moving the treated ovum to the vitrification solution and processing (2 process-6) and placing the ovum in egg freezing The proces...

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Abstract

The present invention relates to a method for the vitrified freezing and thawing of canine ova for somatic cell replication, and more particularly, to: a method for the vitrified freezing and thawingof canine ova; and ova frozen and thawed according to same. Conventional techniques for producing vitrified canine ova are difficult to synchronize due to differences in estrus cycles. Conversely, a method for the vitrified freezing and thawing of canine ova, and ova frozen and thawed according to same, according to the present invention enable collected ova to be synchronized with experiment schedules through a vitrified freezing technique, even when estruses occur on different dates, and thus exhibit a high nuclear transfer and fertilization effect.

Description

technical field [0001] The present invention relates to a method of vitrifying and thawing animal eggs for somatic cell cloning, in more detail, to a method of vitrifying and thawing canine eggs and the frozen-thawed eggs according to the method. Background technique [0002] Somatic cell cloning refers to transplanting the nucleus of the somatic cell to be cloned after removing the nucleus of the egg, thereby preparing a cloned organism having the same genetic information as the somatic cell. Somatic cell cloning is an epoch-making technology that can produce living organisms through somatic cell nuclear transfer without the fertilization process of egg and sperm. At present, somatic cell cloning technology has been widely used in the production of useful animals, the production of transformed animals for the production of expensive drugs, the cloning of endangered animal species, pet cloning, and regenerative medicine. [0003] Somatic cell cloning requires delicate mecha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12N5/075
CPCA01N1/0221A01N1/021A01N1/0268A01N1/0284C12N15/877A61D19/04C12N5/0609A01N1/0205
Inventor 金敏奎南润晳朴江善池国斌李智慧
Owner 艾牧克生物技术有限责任公司
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