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Method for ferment production of arachidonic acid grease with low-content nervonic acid and EPA

A technology of arachidonic acid and nervonic acid, which is applied in the biological field, can solve the problems of low strain activity, identity, and high cost, and achieve the effects of simple fermentation conditions, favorable fermentation process, and improved production capacity

Inactive Publication Date: 2008-04-02
武汉麦可得生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The arachidonic acid products currently on the market are mainly extracted from deep-sea fish oil. Due to the limitation of raw materials, the content of arachidonic acid is low and very unstable, and the output is low and the cost is high, so it cannot be mass-produced
In recent years, Martek Biosciences Co., Ltd. has applied for a patent in China, publication number: CN1175976A (name: arachidonic acid and its production and use method), which uses Mortierella alpina species and is produced by fermentation. However, there are still disadvantages such as low strain activity, low oil yield, and low arachidonic acid production capacity.
Wuhan Xene King Bioengineering Co., Ltd. has applied for a patent in China, publication number: CN1362522A (name: method for producing arachidonic acid-containing oil with ion beam bioengineering mutagenic bacteria), which uses ion implantation method for mutagenesis screening A strain of Mortierella alpina is produced by fermentation, but because the ion implantation method is used to mutate the bacteria, it is not recognized by consumers
And at present all published patent literatures all do not limit the content of the nervonic acid in the produced arachidonic acid mixed oil, but research shows that nervonic acid has adverse effects on the development of infants and young children, and foreign consumer groups have already expressed concern for peanuts. Limit requirements for the content of nervonic acid in tetraenoic acid mixed oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 (shake flask fermentation):

[0027] 1) Activation of strains:

[0028] Preparation of activated medium: The activated medium is based on the potato dextrose agar (PDA) medium, with an additional nitrogen source added. The formula for the activated medium is: 200g potatoes, 1000ml water, 10g glucose, 17g agar, 5g yeast extract ;

[0029] Specifically: Weigh 200g of potatoes, wash, peel and chop them, add 1000ml of water to boil for half an hour, filter with gauze, add 10g of glucose, 17g of agar and 5g of yeast extract, fully dissolve, filter with hot gauze, pack into test tubes, The test tube is about 5-10ml (depending on the size of the test tube), sterilized with 15 pounds of steam (121°C) for about 20 minutes, then take out the test tube and place it on an inclined plane, and store it after cooling.

[0030] Mortierella isabellina was cultured for strain activation on the slope of the activation medium, at 25°C, with an incubator humidity of 40%, and ...

Embodiment 2

[0039]Embodiment 2 (10L tank fermentation):

[0040] 1) Activation of strains:

[0041] Preparation of activated medium: The activated medium is based on potato dextrose agar (PDA) medium, with additional nitrogen source added. The formula for activated medium is: 200g potatoes, 1000ml water, 15g glucose, 18g agar, 7g yeast powder ;

[0042] Specifically: Weigh 200g of potatoes, wash, peel and chop them, add 1000ml of water to boil for half an hour, filter with gauze, add 15g of glucose, 18g of agar and 7g of yeast powder, fully dissolve and filter with hot gauze, pack into test tubes, The test tube is about 5-10ml (depending on the size of the test tube), sterilized with 15 pounds of steam (121°C) for about 20 minutes, then take out the test tube and place it on an inclined plane, and store it after cooling.

[0043] Mortierella isabellina was cultured for strain activation on the slope of the activation medium, at 28° C., and the humidity of the incubator was 40%, and cult...

Embodiment 3

[0052] Embodiment 3 (50L tank fermentation):

[0053] 1) Activation of strains:

[0054] Preparation of activated medium: The activated medium is based on the potato dextrose agar (PDA) medium, with an additional nitrogen source added. The formula for the activated medium is: 200g potatoes, 1000ml water, 20g glucose, 20g agar, 10g peanut powder ;

[0055] Specifically: Weigh 200g of potatoes, wash, peel and chop them, add 1000ml of water to boil for half an hour, filter with gauze, add 20g of glucose, 20g of agar and 10g of peanut powder, dissolve them fully, filter with gauze while hot, and divide into test tubes. The test tube is about 5-10ml (depending on the size of the test tube), sterilized with 15 pounds of steam (121°C) for about 20 minutes, then take out the test tube and place it on an inclined plane, and store it after cooling.

[0056] Mortierella isabellina was cultured for strain activation on the slant of the activation medium, at 25°C, with a humidity of 50% ...

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PUM

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Abstract

The invention relates to a method for producing arachidonic acid oil (AA oil) low in nervonic acid and eicosapentaenoic acid (EPA). The method provided by the invention adopts the mortierella isabelina as the original strain, which undergoes strain activation, preparation of the strain in a shake bottle, and fermentation, then the bacteria are harvested, and the AA oil low in nervonic acid and eicosapentaenoic acid is extracted from the bacterium. With the eicosapentaenoic acid as the original strain for fermentation, the capability of oil production can be improved for 1-3 times, the yield of AA can be raised for 1-3 times; the invention has the simple fermentation condition with controllable process, and capability to produce the mixed oil of AA with high quality which is low in nervonic acid and EPA.

Description

technical field [0001] The invention belongs to biotechnology, and in particular relates to a method for producing arachidonic acid oil containing low nervonic acid and low eicosapentaenoic acid (EPA). Background technique [0002] Arachidonic acid, English is Arachidonic acid, referred to as AA, is 5,8,11,14-eicosatetraenoic acid, it is an essential fatty acid for human body, it belongs to n-6 series of polyunsaturated fatty acids, shorthand For 20:4 (n-6), a large number of studies have shown that the lack of polyunsaturated fatty acids in the human body will cause body dysfunction and lead to certain diseases, and supplementing polyunsaturated fatty acids can prevent or treat these diseases, such as infants lacking arachidonic acid Acid and DHA can cause permanent mental retardation and visual impairment. Children aged 6-12 have symptoms such as itchy skin, dry eyes, inattention in class, and decreased math performance, which are obviously related to the low content of ar...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/14C12R1/645
Inventor 鲁伟岳传静岳传军吴志伟
Owner 武汉麦可得生物技术有限公司
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