Anti-viral agent

An antiviral agent, hepatitis virus technology, applied in the direction of antiviral agent, antitumor drug, digestive system, etc., can solve problems such as no specific proof

Inactive Publication Date: 2008-03-26
MITSUBISHI TANABE PHARMA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as to whether HGF itself has the function of directly eliminating hepatitis virus, there is no concrete proof so far.

Method used

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  • Anti-viral agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] (Example 1) Effect of HGF Addition in HCV-Replicon Cells Relative to Inhibition of HCV-Replicon Replication of IFN

[0073] (Experimental materials and methods)

[0074] As a cell line for replicating HCV in vitro, a clone of HCV-replicon cells, #5-15 (purchased from ReBLikon GmbH), was used using the human hepatoma cell line Huh7 as a mother line. Suspend #5-15 in Dulbecco's Eagle's (Dalbetsukoi-イ-グル) MEM medium containing 2% bovine fetal serum, dilute at 1.5×10 4 / 100μl / well (well) was seeded on a 96-well plate. As a blank, a well (Day 0 ) in which cells were not seeded and only a medium was added was set. at 5% CO 2 After culturing in a humid incubator at 37°C for one day and night under gas, 50 μl of human recombinant IFNα (manufactured by BIOMEDICAL LABORATORIES, Cat. No. 11105-1, lot No. #2122) and / or human Recombinant HGF (manufactured by our company, Lot. 920629). The final concentration of IFNα is 0, 0.1, 0.3 international unit (IU) / ml, the final concentra...

Embodiment 2

[0081] (Example 2) Effects of IFN, HGF or combinations thereof on the proliferation of HCV-replicon cell lines

[0082] (Experimental materials and methods)

[0083] In order to investigate whether the decrease in the replication amount of HCV-replicon confirmed in Example 1 was due to the decrease in the number of cells itself, the following experiment was performed.

[0084] Suspend #5-15 in Dalubetsu Koi-Gru MEM medium containing 2% bovine fetal serum, and use 1.5×10 4 / 100μl / well seeded on a 96-well plate. As a blank, a well (Day 0 ) in which cells were not seeded and only a medium was added was set. at 5% CO 2 After culturing in a humid incubator at 37°C for one day and night under gas, 50 μl of human recombinant IFNα (manufactured by BIOMEDICAL LABORATORIES, Cat. No. 11105-1, lot No. #2122) and / or human Recombinant HGF (manufactured by our company, Lot. 920629). The final concentration of IFNα was 0, 0.1, 0.3 international unit (IU) / ml, the final concentration of HG...

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Abstract

An antiviral agent consisting of hepatocyte growth factor (HGF) or an agonist of the HGF receptor, and a medicament for prophylactic and / or therapeutic treatment of a disease caused by hepatitis C virus comprising using said agent simultaneously or separately with other anti-viral agent.

Description

technical field [0001] The present invention relates to an antiviral agent. Background technique [0002] Hepatitis virus type C (Hepatitis virus type C, hereinafter referred to as "HCV") is becoming the leading cause of hepatitis, liver cirrhosis, and liver cancer in Japan. It is estimated that there are about 1.5 million patients with chronic hepatitis and about 300,000 patients with liver cirrhosis in Japan, 70%-80% of which are caused by HCV. HCV is infected through blood, so the infection caused by blood transfusion can be almost ruled out by screening the blood transfused. Become a breeding ground for new patients with chronic hepatitis. [0003] Since HCV only infects humans and chimpanzees, and there is no suitable infective or reproducing strain in vitro, the development of drugs for hepatitis C has been slow. Various medicines have been used in the treatment, but so far only various interferon (hereinafter abbreviated as "IFN") preparations and ribavirin are eff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K31/7056A61K38/21A61K45/00A61K45/06A61P31/14A61P43/00
CPCA61K38/1833A61K38/212A61K31/7056A61K45/06A61P1/16A61P31/12A61P31/14A61P35/00A61P43/00A61K2300/00
Inventor 石井健久伊丹清马
Owner MITSUBISHI TANABE PHARMA CORP
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