Radix pseudostell adventitious root inducing and tissue culturing method

A tissue culture and Radix ginseng technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., to achieve the effects of fast growth, simple harvest and high efficiency

Inactive Publication Date: 2008-03-19
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After checking the existing technology, there is no literature report and application on the tissue culture of Pseudostellariae adventitious root and its active ingredient production method, and the only suspension cell culture system of Pseudostellariae only grows 5 times in 30 days

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take Pseudostellaria fibrous root explants, soak and sterilize them in 75% ethanol for 30 seconds, rinse with sterile water for 5 times, then soak and disinfect with 1% sodium hypochlorite (available chlorine content) for 20 minutes, wash with sterile water for 5 times, and put them under aseptic conditions. Cut fibrous root explants into sections (1 cm), place them on MS solid medium (pH 5.8) containing 1.0 mg / L 2,4-dichlorophenoxyacetic acid and 0.1 mg / L kinetin, and store them under sterile conditions at 20°C. Fast-growing, pale yellow callus was obtained after 28 days of light induction. Carefully peel off the callus and continue culturing on the same medium. The expanded callus was placed in 1 / 2 MS medium supplemented with 5 mg / L indolebutyric acid, 0.1 mg / L naphthaleneacetic acid and 0.1 mg / L kinetin Adventitious roots were induced on solid medium for 28 days at 20°C under sterile and dark conditions. Take the above induced adventitious roots, transfer them to 1 / ...

Embodiment 2

[0026] Take Pseudostellaria stem explants, soak them in 75% ethanol for 30 seconds, wash them with sterile water for 5 times, then soak them with 1% sodium hypochlorite (available chlorine content) for 15 minutes, wash them with sterile water for 5 times, and put them under sterile conditions. The stem explants were cut into sections (1 cm), placed on MS solid medium (pH 5.8) containing 2.0 mg / L 2,4-dichlorophenoxyacetic acid and 0.2 mg / L kinetin, at 22°C without Bacteria were induced in the dark for 25 days to obtain fast-growing, pale yellow callus. Carefully peel off the callus and continue culturing on the same medium. The expanded callus was placed on 1 / 2 MS solid medium supplemented with 3 mg / L indolebutyric acid and 0.3 mg / L kinetin, at 22 Induce adventitious roots for 25 days under sterile and light-proof conditions at ℃. Take the adventitious roots induced above, transfer them to 1 / 2MS liquid medium containing 3mg / L indolebutyric acid and 1mg / L naphthaleneacetic acid...

Embodiment 3

[0029] Take Pseudostellaria stem explants, soak them in 75% ethanol for 30 seconds, wash them with sterile water for 5 times, then soak them with 1% sodium hypochlorite (available chlorine content) for 15 minutes, wash them with sterile water for 5 times, and put them under sterile conditions. The stem explants were cut into sections (1 cm), placed on MS solid medium (pH 5.8) containing 1.5 mg / L 2,4-dichlorophenoxyacetic acid and 0.1 mg / L kinetin, at 25°C without Bacteria were induced in the dark for 18 days to obtain fast-growing, pale yellow callus. Carefully peel off the callus and continue culturing on the same medium. The expanded callus was placed on 1 / 2 MS solid medium supplemented with 2 mg / L indolebutyric acid and 0.1 mg / L kinetin, at 25 Induce adventitious roots for 25 days under sterile and light-proof conditions at ℃. The adventitious roots induced above were taken and transferred to 1 / 2 MS liquid medium containing 2 mg / L indolebutyric acid and 1 mg / L naphthalenea...

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PUM

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Abstract

The invention provides a radix pseudostell adventitious root induction method and a radix pseudostell adventitious root tissue culture method of the medicinal technical field, which comprises that (1) a poisoned radix pseudostell explant is cancelled, and is inoculated on a callus tissue induction culture base after being cut under the bacteria-free condition, and the callus tissue is induced under the bacteria-free light-free condition; (2) the callus tissue that is grown fast with light-yellow color is fetched to be arranged on a 1 / 2MS solid culture base comprising one or more plant hormones of the seradix, the rhodofix and the kinetin to be induced to form a adventitious root under the bacteria-free light-free condition; (3) liquid culture of the radix pseudostell adventitious root-the obtained adventitious root is arranged in a 1 / 2MS liquid culture base comprising one or more plant hormones of the seradix, the rhodofix and the kinetin to be cultivated. The method of the invention is simple and the efficiency is high; the adventitious root induction rate of the explant of the stem and the leaves of the radix pseudostell reaches more than 90 percent. The adventitious root grows fast with less inoculation and the growth rate can reach more than 46 times after being cultured in a bottle for 18 days.

Description

technical field [0001] The invention relates to a cultivation method in the technical field of plants, in particular to a method for inducing and tissue-culturing adventitious roots of heterophylla. Background technique [0002] Pseudostellaria heterophylla (Miq.) Pax exPax ex Hoffm is the dried root of Pseudostellaria heterophylla (Miq.) Pax exPax ex Hoffm. It is a commonly used tonic traditional Chinese medicine recorded in "Chinese Pharmacopoeia (Fifth Edition)". The function of the lungs is often used clinically to treat spleen deficiency, fatigue, loss of appetite, weakness after illness, deficiency of Qi and Yin, spontaneous sweating and thirst, and dry cough due to dryness of the lungs. Folks are often used as a strong tonic, especially for the treatment of children with spleen deficiency and loss of appetite. Modern chemical and pharmacological studies have shown that Radix Pseudostellaria mainly contains cyclic peptides, triterpenoid saponins, polysaccharides and f...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 贾伟高先富赵爱华
Owner SHANGHAI JIAO TONG UNIV
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