Tissue inner carrying bacterium explant disinfection processing method

Abstract

The present invention relates to a treating method for sterilizing the explants, which have bacteria in tissues. The treating method is the plant material of the explants are implemented with the common treatment and the part of the fringe of the explants whose color changes with the sterilization is cut and then the explants are cut into small pieces of 1 to 3 cm for use; the method is characterized in that the explants are separately transplanted into different culture media of different concentrations; 8 to 10 explants are transplanted in one triangular bottle and the fringe of every explant contact the culture media; the explants are put in a culture room for cultivation. The method of the present invention is characterized by simple operation; the three antibiotics of carbenicllin, streptomycin sulphate and nystatin have obvious effect on the sterilization of the explants which not only effectively constrain the bacteria (100 percent when the three antibiotics are injected with the amount of 500 plus 100 plus 1000mg an hour) but also basically control the growth of fungus.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a treatment method for disinfecting explants carrying bacteria in tissues. Background technique [0002] Tissue culture is to place the plant organs (roots, stems, leaves, etc.), tissues, cells and protoplasts separated from the mother tree in an artificially prepared medium under sterile conditions, and cultivate them into a large number of them under artificially controlled conditions. The whole new plant, the part taken from the parent body for culture is called an explant. Tissue culture has the following advantages: (1) Culture conditions can be artificially controlled. The plant materials used in tissue culture are grown completely under the conditions of artificial culture medium and microclimate environment, which get rid of the adverse effects of four seasons, day and night changes and disastrous climate in nature; and the conditions are uniform, which is extreme...

AI Technical Summary

Problems solved by technology

For these microorganisms living inside the plant tissue (in the plant tissue space or in the plant cells), if the conventional disinfection treatment is used, the purpose of explant disinfection often cannot be achieved; although some fungi have not grown up in the initial stage of tissue culture , but as the culture time increases, bacteria and fungi inside the plant tissue will appear, making tissue culture impossible