Chinese herbal medicine extracting solution used for stevia rebaudiana culture medium and preparation method

Abstract

The invention belongs to the technical field of stevia rebaudiana culture mediums and provides a Chinese herbal medicine extracting solution used for a stevia rebaudiana culture medium and a preparation method. The Chinese herbal medicine extracting solution used for the stevia rebaudiana culture medium is prepared from the following Chinese herbal medicine components: lignum dalbergiae odoriferae, piper longum, pseudobulbus cremastrae seu pleiones, spora lygodii, Bianjiang and mahonia fortunei; the Chinese herbal medicine extracting solution is used for any one of a stevia rebaudiana callus culture medium, a protocorm culture medium, a subculture culture medium, a passage culture medium and a rooting culture medium. All the Chinese herbal medicines are prepared through techniques such as ethanol digestion, boiling and concentration. Aiming at disadvantages of a bacterium-inhibiting measure in existing stevia rebaudiana tissue culture, the Chinese herbal medicines are added into a basic culture medium and the culture medium is enabled to be located in an immune environment, so that pollution caused by microorganisms including external bacteria and the like is prevented, and furthermore, the efficient and lasting bacterium inhibition effect is realized; meanwhile, the resistance of stevia rebaudiana explants is improved, the investment of manpower and material resources is reduced and the cost is reduced. The method is simple to operate and has a good extracting effect.

Description

technical field [0001] The invention belongs to the technical field of stevia culture medium, and in particular relates to a Chinese herbal medicine extract used in stevia culture medium and a preparation method. Background technique [0002] In recent years, stevia tissue culture technology has developed rapidly. In the tissue culture process, there are often microbial contamination problems such as bacteria, resulting in poor quality of stevia tissue culture, low efficiency, and serious nutrition. A problem for people. [0003] The microbial contamination during the stevia tissue culture process mainly comes from two aspects: one is the plant material itself carrying bacteria; the other is caused by the operator and the microorganisms in the air; in addition, the pollution may also be caused by the medium carrying bacteria and the cross contamination of operating tools. caused by pollution. Contaminating microorganisms are divided into two categories: bacteria and fungi....

AI Technical Summary

Problems solved by technology

The above-mentioned measures to prevent microbial contamination in the tissue culture process all have certain effects, but the disadvantage is that each antibacterial link is isolated and cannot form a complete and unified antibacterial chain. If any link in the process is negligent, microorganisms will take advantage of it. , resulting in failure of tissue culture, reducing the quality and efficiency of tissue culture. In addition, through sterilization of the medium, aseptic inoculation, and cultivation in a sterile environment, the purpose of sterilization and antibacterial is achieved through external operations. In these links Once any link is not in place, bacteria and other microorganisms will be infected. Therefore, a measure of continuous antibacterial function is needed to keep the stevia explants in a sterile environment for a long time, and at the same time enhance the internal antibacterial function of stevia explants Ability to resist contamination by foreign microorganisms