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Agarose and hyaluronic acid grafts and preparation method and uses thereof

A technology of hyaluronic acid and agarose, applied in the field of biomedical materials, can solve the problems of limited application of hyaluronic acid, short retention time, easy to degrade, etc., so as to increase added value, accelerate the formation of tissues, and be beneficial to enrichment. The effect of ecological restoration

Inactive Publication Date: 2010-06-16
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, certain properties of hyaluronic acid, such as being easily soluble in water, poor in stability, sensitive to strong acid, strong alkali, heat, free radicals and hyaluronidase, are prone to degradation, and have a short retention time in the human body, which greatly limits Application of Hyaluronic Acid in Biomedicine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, preparation of porous composite scaffold preparation and skin dressing

[0036] Prepare hyaluronic acid and agarose graft, comprise the following steps:

[0037](1) Dissolve agarose in hot water at 90°C with a concentration of 5% by mass, cool down to 60°C, add hydrogen peroxide with a concentration of 30% by mass to make the final concentration reach 1% by volume, and react at a constant temperature at 50°C 15 hours;

[0038] (2) After concentrating the solution obtained in step (1) under reduced pressure, add ethanol equivalent to 4 times the volume of the solution, centrifuge to obtain a precipitate, dissolve the precipitate in water, and repeat the precipitation with ethanol to remove residual hydrogen peroxide. Dissolved, put into freeze-drying machine and freeze-dry to obtain degraded agarose powder;

[0039] (3) The degraded agarose powder was dissolved in hot water at 95° C., cooled to room temperature, and sodium hydroxide solution and epichloro...

Embodiment 2

[0044] Example 2, preparation of adipose tissue engineering scaffold

[0045] Prepare hyaluronic acid and agarose graft, comprise the following steps:

[0046] (1) Dissolve agarose in hot water at 95°C with a concentration of 1% by mass, cool down to 60°C, add hydrogen peroxide with a concentration of 30% by mass to make the final concentration reach 1% by volume, and react at a constant temperature at 60°C 6 hours;

[0047] (2) After concentrating the solution obtained in step (1) under reduced pressure, add ethanol equivalent to 5 times the volume of the solution, centrifuge to obtain a precipitate, dissolve the precipitate in water, and repeat the precipitation with ethanol to remove residual hydrogen peroxide. Dissolved, put into freeze-drying machine and freeze-dry to obtain degraded agarose powder;

[0048] (3) The degraded agarose powder was dissolved in hot water at 90° C., cooled to room temperature, and sodium hydroxide solution and epichlorohydrin were added so th...

Embodiment 3

[0053] Example 3, preparation of targeted drug carrier for controlled release

[0054] Prepare hyaluronic acid and agarose graft, comprise the following steps:

[0055] (1) Dissolve agarose in hot water at 93°C with a concentration of 3% by mass, cool down to 55°C, add hydrogen peroxide with a concentration of 30% by mass to make the final concentration reach 2% by volume, and react at a constant temperature at 55°C 10 hours;

[0056] (2) After concentrating the solution obtained in step (1) under reduced pressure, add ethanol equivalent to 4.5 times the volume of the solution, centrifuge to obtain a precipitate, dissolve the precipitate in water, and repeat the precipitation with ethanol to remove residual hydrogen peroxide. Dissolved, put into freeze-drying machine and freeze-dry to obtain degraded agarose powder;

[0057] (3) The degraded agarose powder was dissolved in hot water at 93° C., cooled to room temperature, and sodium hydroxide solution and epichlorohydrin were...

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Abstract

The present invention relates to hyaluronic acid-agarose graft and its preparation process and application. The hyaluronic acid-agarose graft is prepared through the following steps: dissolving degraded agarose powder in hot water, cooling to room temperature, and adding sodium hydroxide solution and epoxy chloropropane to react under magnetic stirring; repeated vacuum concentrating, water dissolving and alcohol precipitating to eliminate epoxy chloropropane and obtain activated agarose precipitate; dissolving activated agarose powder in hot water, cooling to room temperature, adding hyaluronic acid to produce grafting reaction under magnetic stirring; vacuum concentrating the solution, dialyzing in bag filter for 72 hr to eliminate un-reacted hyaluronic acid, and freeze drying to obtain hyaluronic acid-agarose graft. The hyaluronic acid-agarose graft as one new biomedical material has bioactivity and targeting property from hyaluronic acid, gel forming feature, improved biodegradability and certain stability.

Description

technical field [0001] The invention relates to biomedical materials, in particular to a preparation method and application of agarose and hyaluronic acid grafts. Background technique [0002] Biomedical materials have played an extremely important role in the improvement of modern medical technology. Various drug release systems, tissue engineering scaffolds, artificial organs, medical dressings, etc. made of them involve almost all clinical fields. With the improvement of medical technology, those biomedical materials with single structure and performance can no longer meet the needs of medical development, and the development of new biomedical materials is an urgent need at present. Among them, targeted drug controlled release carrier materials and functional active tissue engineering scaffold materials are important directions for future biomedical material research. [0003] In 1976, Langer and Folkman successfully realized the controlled release of macromolecular drug...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08A61L27/20A61L31/10A61K47/36
Inventor 汤顺清包磊毛萱叶巧巧黄建艳
Owner JINAN UNIVERSITY
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