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Method for producing phytase

A technology of phytase and microbial strains, applied in the field of phytase production, can solve the problems of low content, high cost, long cycle, etc., and achieve the effect of high technology content and cost reduction

Inactive Publication Date: 2010-12-08
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI +1
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  • Description
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Problems solved by technology

[0004] Phytase is also contained in natural crops, such as corn germ, soybean, etc., but its content is low, and the growth cycle of crops is long
Extracting phytase in this way, the cost is too high, the cycle is long, and it is not suitable for industrialization

Method used

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  • Method for producing phytase
  • Method for producing phytase
  • Method for producing phytase

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Embodiment Construction

[0020] First of all, choose vinegar, smoked mackerel (the abandonment after smoking and vinegar in vinegar) is three kinds of badminton in the medium of sorghum, corn, sorghum and corn as the main material;(Aspergillus FICUM), which is purchased at the Institute of Microbiology of the Chinese Academy of Sciences, the bacteria number is: 3.4322.Different fermentation conditions are used to experiment, so as to determine the impact of different fermentation conditions on planting acid enzymes:

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Abstract

The present invention discloses a method for producing microbial phytase. The present invention utilizes vinegar residue as a solid culture medium, and microbial strains can be inoculated to produce microbial phytase, then microbial phytase can be obtained after being fermented. The present invention has the following production technical processes: firstly, the vinegar residue culture medium is put into a container, then the water content of the culture medium is adjusted; secondly, the disinfection is carried on; thirdly, the inoculation is carried on under the condition of asepsis; fourthly, ferment is carried on. The method for producing microbial phytase in the present invention selects the waste -vinegar residue in vinegar production, thus changing waste into valuable, making the microbial phytase manufacturing cost greatly reduced, and resulting the reasonable reuse of the vinegar residue.

Description

Technical field [0001] The present invention involves a method for producing acid enzymes. Background technique [0002] Phosphorus is a must -have mineral element necessary for animal body. In order to meet the needs of the feed and breeding industry, it consumes a lot of expensive phosphate resources.Only a small part of the phosphorus is deposited into the body and animal products, so when the environment, especially the intensive breeding, the pollution of groundwater and soil is very serious. [0003] With the development of biotechnology, people have discovered that acid enzymes have become the most attractive way to solve feed phosphorus problems.By adding acid enzymes in single -gastric animal feed, it can improve the utilization rate of phosphoric phosphoric (or phosphate) in the feed, and reduce the pollution of the environment and water discharge of phosphorus in livestock and poultry excretion.With biotechnology, especially DNA reorganization technology, a large numbe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/16C12N1/14C12R1/66
Inventor 佟建明王志红萨仁娜张琪董晓芳吴莹莹
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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