Double color fluorescent quantitative co-amplified polymerase chain reaction detection kit

Abstract

The invention discloses a reaction reagent box of double color fluorescence batching polymerase chain. Firstly, according to the distinguished DSCR sector sequence of the 21 chromosome and the GAPDH gene sequence of the 16 chromosome, the distinguished primer and the double color Taqman probe are separately synthesized, and then, the synthesis of the new chains are guided separately and simultaneously by the two pairs of primers through 45 PCR circulations in the identical expanded cushioning reacting system, and the altogether expanding of the two pairs of primers is realized. The signal changing situation of the fluorescence obtained because of the degrading of the Taqman probe in the expanding process is inspected when expanding the PCR, and the curve diagrams are obtained, and the Ct value of each gene is obtained. And therefore, the copy number ratio can be calculated, and the ploidy of the 21 chromosomes can be judged according to the ratio of the two gene copy number.

Description

technical field [0001] The invention relates to a double-color fluorescence quantitative co-amplification polymerase chain reaction detection kit, in particular to a double-color fluorescence quantitative polymerase chain reaction in vitro co-amplification PCR kit for chromosome 21 ploidy detection, which belongs to molecular biology Detection and diagnosis field. Background technique [0002] Down syndrome (Down Syndrome, DS), also known as congenital folly, is one of the most common chromosomal abnormality syndromes in live-born fetuses, with an incidence of about 1 / 600-1 / 800. The signs of DS are very diverse, usually involving abnormalities of multiple tissues and organs, but the most prominent and severe clinical manifestation is mental retardation. The main clinical features of the disease are: severe mental retardation, and the phenotype of mental retardation gradually becomes obvious with age, motor development and sexual development are retarded, and there is basica...

AI Technical Summary

Problems solved by technology

The results of a large number of population surveys around the world show that the incidence rate between races is almost the same. There are 3 chromosomes 21 in each somatic cell of Down syndrome, while other autosomes are 2. Lymphoblasts or amniotic fluid exfoliated cells are cultured in vitro and Chromosome preparation observation is a routine method for laboratory testing. Although this method is highly accurate, it is complex and time-consuming, and cannot be automated and high-throughput.