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Human immunodeficiency virus antibody confirmations reagent

A human immunodeficiency, antibody technology, applied in biological testing, material analysis by observing the impact on chemical indicators, material inspection products, etc., can solve the problems of complicated operation methods and long measurement time.

Inactive Publication Date: 2008-01-02
曹健荣 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the domestic diagnosis method for AIDS mainly adopts the ELISA method. This method has high sensitivity and specificity, but its operation method is complicated, the measurement time is long, and certain operation experience and equipment are required. In some areas, especially in remote rural areas, the use of this method is limited, so it is necessary to develop a quick and easy detection reagent

Method used

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  • Human immunodeficiency virus antibody confirmations reagent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] (1) Confirm the preparation and testing of test strips

[0123] with coating buffer. According to a certain concentration, the detection line and the quality control line are uniformly and neatly fixed on the mixed cellulose membrane with a flow test film scratcher.

[0124] Confirm the dryness of the test strip

[0125] After the coating was completed, it was placed on an ultra-clean workbench and air-dried at room temperature for 2 hours. Store in a dry environment at room temperature (16-25°C, relative temperature not higher than 45%).

[0126] semi-finished product inspection

[0127] The national reference product of HIV antibody confirmation reagent was used for verification.

[0128] The compliance rate of HIV antibody positive reference products is 10, and the required positive compliance rate is 10 / 10.

[0129] The HIV antibody negative reference product conformity rate was 12, and the required negative conformity rate was 12 / 12.

[0130] The coincidence ...

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Abstract

The invention relates to a kind of affirmation regent for antibody of human immunodeficiency virus, and the diagnosing regent consists of HIV-I type specificity protein gp160, gp120, gp41, p66, p24, mixed fiber film which is encysted by HIV-II specificity protein gp36, enzyme conjugates and other component, and the principle of indirect process is provided for the affirmation of antibody of HIV-I and HIV-II type.

Description

Technical field: [0001] The invention relates to a medical diagnostic reagent, in particular to a human immunodeficiency virus antibody confirmation reagent. It is composed of mixed cellulose membrane, enzyme conjugates and other components independently coated with sex protein gp36, and is used for the confirmation of HIV-I and HIV-II antibodies by applying the principle of indirect method. Background technique: [0002] The widespread and harmfulness of the spread of human acquired immunodeficiency syndrome (AIDS) has become a public health and social hot issue of global concern. The spread of AIDS in our country has entered a period of rapid growth. In addition to more HIV-infected people in economically developed coastal areas, in some areas with underdeveloped economies and poor health care, especially rural areas, due to the continuous flow of labor exports The increase and the number of HIV-infected people from plasma harvesting have put a lot of pressure on my countr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/78G01N33/549G01N33/53
Inventor 曹健荣张誌王宇帆王建丽张海燕
Owner 曹健荣
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