Method for preparing 2 type diabetes rat model
A type 2 diabetes and rat model technology, applied in preparations for in vivo experiments, pharmaceutical formulas, animal feed, etc., can solve the problem of long modeling time, failure to reach clinical diagnosis of diabetes, and low fasting blood sugar in T2DM rat models and other problems, to achieve the effect of high model success rate and shortened molding time
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[0033] 1. Experimental materials
[0034] 1. Main medicines and kits
[0035] Streptozotocin (STZ), American Sigma Company;
[0036] Citrate, Boster;
[0037] Serum triglyceride (TG) test kit, serum cholesterol (Tch) test kit, Shanghai Rongsheng Biotechnology Co., Ltd.;
[0038] Plasma insulin kit (radioimmunoassay), French CIS company;
[0039] Glycosylated hemoglobin (microcolumn method) assay kit, Ningbo Cicheng Biochemical Reagent Factory;
[0040] Reagents related to Masson staining, Department of Pathology, Shanxi Medical University;
[0041] Blood glucose test strips, American Johnson & Johnson;
[0042] Urine glucose test strips, Guilin Zhonghui Biotechnology Co., Ltd.
[0043] 2. Main instruments
[0044] Johnson & Johnson sure step plus blood glucose meter, Johnson & Johnson;
[0045] UV755B ultraviolet-visible spectrophotometer, Shanghai Analytical Instrument General Factory;
[0046] LD4-2A desktop centrifuge, Beijing Medical Centrifuge Factory;
[0047] ...
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