Fluorescent quantitative detection device for PCR

A fluorescence quantitative detection and fluorescence signal technology, which is applied to the measurement/inspection of microorganisms, fluorescence/phosphorescence, biochemical equipment and methods, etc. Detect problems such as background noise to achieve the effect of reducing background noise, reducing inaccurate positioning, and overcoming fluctuations

Inactive Publication Date: 2007-12-12
TONGJI UNIV
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  • Abstract
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Problems solved by technology

This kind of movement will increase the background noise of the detection, make the detection data fluctuate, and also cause the fixture to loosen af...

Method used

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  • Fluorescent quantitative detection device for PCR

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Embodiment Construction

[0024] Below in conjunction with accompanying drawing and embodiment the utility model is described further.

[0025] A PCR fluorescence quantitative detection device whose model is PG2000, this fluorescence quantitative detection device is vertically provided with a bearing 7 in the center of the housing 1, a horizontal circular loading plate 3 is fixed on the upper end of the bearing, and the connection Located at the center of the circular loading tray; the lower part of the bearing is connected with a drive motor 6, the driving motor is a stepping motor, driven by the stepping motor, the bearing 7 can drive the circular loading tray 3 according to the stepping The motor rotates at the preset number of steps. The edge of the circular object tray is inclined upwards to form a circumferential inclined surface with an inclination angle of 45° with the horizontal plane where the object tray is located. A plurality of test tube holes distributed in a circle are arranged on the ...

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Abstract

The PCR check device comprises the shell, several objects carrying plate for sample tubes inside the shell, emitting light activating the light signal and light collecting post fixed on the shell, drive motor driving the object carrying plate and the relative movement of the sample tubes with the emitting light and the light collecting post. It can ensure the optical component fastness for a long time, with reduced testing background noise, getting rid of the wave in testing, accuracy and collection of optical path for several samples at the same time.

Description

technical field [0001] The utility model relates to a fluorescence detection device, in particular to a fluorescence quantitative detection device for polymerase chain reaction (Polymerase Chain Reaction, PCR). Background technique [0002] In the past ten years, in the diagnosis and treatment of infectious diseases, the polymerase chain reaction (PCR) fluorescence quantitative method can quickly detect the existence of pathogenic viruses such as SARS virus and avian influenza virus and perform quantitative analysis. The detection device has been greatly developed. Compared with other assay methods based on immune response, the PCR fluorescent quantitative detection device has the outstanding advantage of detecting viruses during the incubation period. In epidemic areas or foci, early and rapid diagnosis of pathogenic viruses can gain valuable time for controlling the spread of diseases . It can not only be used to measure changes in the number of pathogenic viruses in con...

Claims

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Application Information

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IPC IPC(8): G01N21/64C12Q1/00
Inventor 朱文斯罗光弼吕立夏
Owner TONGJI UNIV
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