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Immune chromatography test paper for inspecting legionella pneumophilia antibody and its production

A technology of immunochromatographic test paper and Legionella pneumophila, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of difficult standardization of experimental systems and cumbersome operation steps, and achieve rapid sample processing, broad application prospects, and sensitive Highly specific and specific effects

Inactive Publication Date: 2007-11-21
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But in practice, the use of these methods has exposed some common disadvantages, such as: the experiment must be carried out in a special laboratory; power and special instruments are required; operators must be specially trained; the experimental system is not easy to standardize. The results are credible, and it is necessary to conduct multiple pre-tests and set up multiple controls; the test operation steps are cumbersome and need at least 2 hours

Method used

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  • Immune chromatography test paper for inspecting legionella pneumophilia antibody and its production

Examples

Experimental program
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Embodiment 1

[0034] Embodiment 1, the preparation of the immunochromatographic test paper that detects Legionella pneumophila antigen

[0035] 1. Preparation of specific antibody against serotype 1 antigen of Legionella pneumophila

[0036] 1. Preparation of Legionella pneumophila serum type 1 antigen

[0037] Inoculate the serotype 1 strain of Legionella pneumophila (purchased from the strain library of the Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, preservation number 510101) on BCYE plates, and place them at 37°C, 5% CO 2 Cultivate in an incubator, observe the colony shape on the plate, pick a typical single colony, transfer it to a BCYE slope, and place it at 37°C, 5% CO 2 Cultivate in the incubator for 3-5 days. Wash the cultured slant with sterile PBS (0.01M pH7.2) to remove the bacterial lawn, centrifuge at 8000rpm for 25 minutes, discard the supernatant, and collect the bacterial precipitate; each gram of the wet weight of the bacterial cell...

Embodiment 2

[0056] Embodiment 2, the detection of Legionella pneumophila antigen and cross test with other relevant bacteria

[0057] 1. Detection of different serotypes of Legionella pneumophila antigens

[0058] The preparation method of Legionella pneumophila serotype 1 antigen in Step 1 of Example 1 is also applicable to the preparation of other serotype antigens of Legionella pneumophila. Bacterial serum types 1, 2, 3, 4, 5, 6, 7, 9, and 10 strains were prepared according to the method of Example 1. Antigens were diluted to concentrations of 100 μg / mL, 10 μg / mL, 1 μg / mL, and 100 ng / mL, respectively , 10ng / mL sample detection solution for use.

[0059]Get the test kit of the immunochromatographic test paper that the detection of Legionella pneumophila antigen of the present invention is equipped with that embodiment 1 prepares, add above-mentioned sample detection solution 3 drops (about 150ul) respectively in the sampling port, begin to observe the result after 2 minutes, 15 Minute...

Embodiment 3

[0065] Embodiment 3, laboratory assessment

[0066] 1. Sample preparation

[0067] A total of 10 strains of bacteria were used in the experiment, including 9 strains of Legionella pneumophila (strain number: 510101-5101010, purchased from the Microbial Testing Research Center of the Institute of Microbial Epidemiology, Academy of Military Medical Sciences), 1 strain of Yersinia pestis (strain number: 410050, purchased from the Microbiological Testing Research Center, Institute of Microbial Epidemiology, Academy of Military Medical Sciences). The above-mentioned bacteria were inoculated into their respective appropriate medium, and the antigens were extracted after cultured under different conditions. The concentration is 100μg / mL, 10μg / mL, 1μg / mL, 100ng / mL and 10ng / mL, and the concentration of Yersinia pestis F1 antigen is 100μg / mL, which is used as the detection solution for later use.

[0068] 2. Experimental method

[0069] The Legionella pneumophila antigen rapid detect...

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Abstract

An immune chromatographic test paper used for detecting antigen of legionella pneumophilia consists of sample pad, gold label pad of antibody label colloidal gold probe containing serum antigen resisting legionella pneumophilia, nitric acid fiber membrane and water absorption pad. It is featured as enveloping detection line containing antibody and quality control line containing anti-antibody on nitric acid fiber membrane and setting detection line to be separated from quality control line.

Description

technical field [0001] The invention relates to a test paper for detecting Legionella pneumophila antigen and a preparation method thereof, in particular to an immunochromatographic test paper for detecting Legionella pneumophila antigen and a preparation method thereof. Background technique [0002] Legionella was discovered in 1976. At that time, American veterans gathered in Philadelphia, during which a severe pneumonia of unknown cause broke out. 149 participants were infected and 34 died. A new bacterium was isolated from the lung tissue of the deceased, named Legionella. At present, there are 43 species and 65 serotypes of Legionella that have been discovered, among which Legionella pneumophila (LP) is the most closely related to human diseases. Studies have shown that 80% to 85% of Legionella pneumonia Caused by LP, 15 serotypes of LP have been found. [0003] Legionnaires' disease (LD) is an acute bacterial respiratory infectious disease caused by Legionella infec...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/532
Inventor 端青汪黎李艳柏长青朱虹檀华何君左婷庭宋立华
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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