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Composite enzyme sensitive film containing Zn-VIA-family semiconductor nano-particle, its production and use

A nanoparticle and sensitive membrane technology, applied in the field of enzyme bioengineering, can solve the problems of biological toxicity, affecting the performance of enzyme electrodes, etc., and achieve the effects of increasing reaction rate, fast conversion, and reducing resistance

Inactive Publication Date: 2010-05-12
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The reported semiconductor nanoparticles used in biosensors are mainly cadmium-containing materials. Without complicated treatment, these materials will have biological toxicity, which will affect the performance of enzyme electrodes and their application in vivo.

Method used

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  • Composite enzyme sensitive film containing Zn-VIA-family semiconductor nano-particle, its production and use
  • Composite enzyme sensitive film containing Zn-VIA-family semiconductor nano-particle, its production and use
  • Composite enzyme sensitive film containing Zn-VIA-family semiconductor nano-particle, its production and use

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Experimental program
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Effect test

Embodiment 1

[0055] The first step: prepare nano-zinc oxide sol solution by co-precipitation method:

[0056] (1). Prepare the isopropanol solution of zinc acetate and sodium hydroxide respectively, wherein, the molar concentration of zinc acetate and sodium hydroxide is respectively 7 * 10 -4 mol / L and 3.6×10 -2 mol / L.

[0057] (2). Preparation of nano-zinc oxide sol solution; the zinc acetate solution and sodium hydroxide solution of step (1) are respectively added to the reaction vessel and mixed, wherein the mol ratio of zinc acetate and sodium hydroxide is 1: 2.5, mixed The solution was reacted at 75°C for 1 hour to obtain a nano-zinc oxide sol solution with a particle size of 60nm and a molar concentration of 6×10 -4 mol / L.

[0058] The second step: preparation of polymer gel solution:

[0059] Polyvinyl butyral, a polymer substance, was dissolved in ethanol, so that the concentration of the solution was 2% by weight.

[0060] The third step: preparation of composite oxidase fun...

Embodiment 2

[0064] The first step: prepare nano-zinc sulfide sol solution by co-precipitation method:

[0065] (1). Prepare the secondary aqueous solution of zinc sulfate and sodium sulfide respectively, wherein, the molar concentration of zinc sulfate and sodium sulfide is respectively 5 * 10 -4 mol / L and 1.2×10 -2 mol / L.

[0066] (2). Preparation of nano-zinc sulfide sol solution; the zinc sulfate solution and sodium sulfide solution of step (1) are added to the reaction vessel and mixed respectively, wherein the mol ratio of zinc sulfate and sodium sulfide is 1: 1.5, and the mixed solution is React at 35°C for 2 hours to obtain a nano-zinc sulfide sol solution with a particle size of 40nm and a molar concentration of 3×10 -4 mol / L.

[0067] The second step: preparation of polymer gel solution:

[0068] The macromolecular substance chitosan is dissolved in secondary water, so that the weight percent concentration of the solution is 0.5%.

[0069] The third step: preparation of comp...

Embodiment 3

[0073] The first step: Prepare nano zinc selenide sol solution by co-precipitation method:

[0074] (1). Prepare the secondary aqueous solution of zinc nitrate and sodium selenite respectively, wherein, the molar concentration of zinc nitrate and sodium selenite is respectively 4.2 * 10 -4 mol / L and 2×10 -3 mol / L.

[0075] (2). Prepare nano-zinc selenide solution; the zinc nitrate solution and sodium selenite solution of step (1) are respectively added to the reaction vessel and mixed, wherein the mol ratio of zinc nitrate and sodium selenite is 1: 0.5 , the mixed solution was reacted at 220°C for 15 hours to obtain a nano-zinc selenide sol solution with a particle size of 122nm and a molar concentration of 5×10 -4 mol / L.

[0076] The second step: preparation of polymer gel solution:

[0077] Polyurethane, a polymer substance, is dissolved in hexane, so that the weight percent concentration of the solution is 1%.

[0078] The third step: preparation of composite oxidase f...

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Abstract

A high-sensitivity and stability composite enzyme functional sensitive film containing Zn-VIA family semiconductor nano-grain, its preparation and use are disclosed. The process is carried out by mixing Zn-VIA family semiconductor nano-sol with oxidizing enzyme solution, adding into polymer gel solution, agitating, adding into glutaric dialdehyde, cross-linking treating, and coating mixed solutiononto solid carrier surface to form into composite enzyme functional sensitive film containing Zn-VIA family semiconductor nano-grain. The proportion of polymer Zn-VIA family semiconductor nano-grainis 1:3.26X10-7-1:8.15X10-1, it contains oxidizing enzyme 0.1-120 active units, the grain size of Zn-VIA family semiconductor is 1-200 nm. It saves enzyme usage and has better performances. It can be used for biological sensor, biological separating film and enzyme biological catalytic engineering.

Description

technical field [0001] The invention belongs to the technical field of enzyme bioengineering, in particular to a complex enzyme function-sensitive film containing Zn-VIA family semiconductor nanoparticles with high sensitivity and high stability, its preparation method and application. Background technique [0002] Products manufactured by enzyme bioengineering are widely used in human body and industry. For example, glucose biosensors assembled by glucose oxidase can be used to measure human blood sugar, industrial wastewater, sugar in fermentation or beverages. Biosensors have very obvious advantages, such as: high specificity, short-term low-cost analysis, no special requirements for analytical substances, safe operation, convenient on-site determination, etc., and many achievements have been made in many countries. At present, the research on biosensors is developing toward high performance, miniaturization, and entering into the family. It is a high technology that is g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/40C12Q1/26G01N27/327
Inventor 唐芳琼任湘菱孟宪伟陈东
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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