Ruminant staphylococcus separation, bacteria propagation and capsular polysaccharide expression culture medium

Abstract

The invention discloses a separating, enriched and capsular polysaccharide expressing medium of ruminant staphylococcus, which comprises the following steps: choosing milky protein hydrolysate, protease leather, tryptone, soy peptone, lactin, common salt, dipotassium hydrogen phosphate, magnesium sulfate, ferrosi sulfas, L-cysteine hydrochlorate, rabbit brain heart drench and double distilled water as raw material; shearing fresh rabbit brain and heart; grinding; homogenizing; soaking with double distilled water under 4 deg. c; boiling for 15 min; filtering with filter paper; compensating water; getting the rabbit brain heart drench. This invention possesses strong ability of bacterial maintain and passage toxic force, which is fit for separating staphylococcus.

Description

1. Technical field [0001] The invention relates to a bacterial culture medium, in particular to a ruminant staphylococcus isolation, enrichment and capsular polysaccharide expression culture medium. 2. Background technology [0002] Staphylococcus is widely distributed in nature, and pathogenic Staphylococcus often causes various suppurative diseases, sepsis or sepsis in humans and animals. Among them, Staphylococcus aureus, Staphylococcus mimicus and other pathogenic bacteria are common pathogens. Traditional vaccines prepared by using live or inactivated bacterial vaccines, isolated peptide sugars, toxoids, adhesins, etc. have no significant effect on the prevention and treatment of staphylococcal infections. The reason for the ineffectiveness of traditional vaccines is that most pathogenic strains have a capsule (mucus layer / microcapsule). Capsular polysaccharides have anti-phagocytic activity and prevent neutrophils from recognizing antibodies to staphylococcal cell wa...

AI Technical Summary

Problems solved by technology

The defect of these mediums is that after staphylococci are isolated, the capsular polysaccharide as the main immunogen and virulence factor is expressed at a low concentration or even not expressed, and disappears after 2-4 generations of continuous passage, resulting in a rapid decline in the virulence of pathogenic bacteria, even if Virulence cannot be restored by returning this animal

However, in the process of production and scientific research, a culture medium that can still maintain its virulence after isolation, enrichment and continuous passage of staphylococci is required, which can provide support for strain preservation, passage, vaccine protection test, and capsular polysaccharide extraction. Therefore, currently The culture medium does not meet the technical requirements

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