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Selenium-rich and chromium-rich Saccharomyces cerevisiae, selenium-rich and chromium-rich yeast product and their production process

A technology of Saccharomyces cerevisiae and chromium yeast, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as high production costs and complex processes

Active Publication Date: 2007-10-03
ANGEL NUTRITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pure selenium-enriched yeast and chromium-enriched yeast need to be produced separately, the process is more complicated, and correspondingly higher production costs are required

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: the acquisition of yeast strain

[0015] The selenium-rich chromium Saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen Z1.1) used in the present invention has carried out biological preservation on October 25, 2005 in China Center for Type Culture Collection (CCTCC, Wuhan University, zip code 430072), and the preservation number is CCTCC M 205123. It is composed of selenium-enriched yeast strain S205 (referring to the patent application titled "a selenium-enriched Saccharomyces cerevisiae, a selenium-enriched yeast product and its production method" submitted by the applicant at the same time as this application) and chromium-enriched yeast strain C460 ( See the patent application with the title of "A Chromium-enriched Saccharomyces cerevisiae, a chromium-enriched yeast product and its production method" submitted at the same time as this application) which was bred through protoplast fusion technology. The specific implementation steps are as foll...

Embodiment 2

[0021] Embodiment 2: produce selenium-enriched chromium yeast product

[0022] The production process of selenium-enriched chromium yeast: slant culture → primary liquid seed cultivation → secondary liquid seed cultivation → fermenter seed cultivation → fermenter cultivation → separation → drying → packaging. The specific process is as follows:

[0023] 1) Slant strain culture: Inoculate the selenium-rich chromium yeast strain to a sugar content of 5% by weight, a selenium (selenite) concentration of 1 μg / ml, chromium (chromium trichloride) 1 μg / ml, pH Wort solids slants with a value of 4 were incubated at 26°C for 48 hours.

[0024] 2) First-level liquid seed culture: the yeast strain inoculation of the slant is inoculated into 1~6 rings to be equipped with 500ml of sugar content of 5% by weight, selenium (selenite) concentration of 1 μg / ml, chromium (trichloride) Chromium) 1 μg / ml, pH value of 4 wort special culture bottle, at 26 ℃ for 48 hours.

[0025] 3) secondary liqu...

Embodiment 3

[0031] Embodiment 3: produce selenium-enriched chromium yeast product

[0032] The production process of selenium-enriched chromium yeast is the same as embodiment 2, and concrete technology is as follows:

[0033] 1) Slant strain culture: inoculate selenium-enriched chromium yeast strains into a medium with a sugar content of 25%, a selenium (selenate) concentration of 1000 μg / ml, a chromium (chromium acetate) of 1000 μg / ml, and a pH value of 6.5. Wort solid slants were incubated at 36°C for 18 hours.

[0034] 2) First-level liquid seed culture: inoculate 1 to 6 rings of yeast strains on the slant to be equipped with 500ml sugar content of 25% by weight, selenium (selenate) concentration of 1000 μg / ml, chromium (chromium acetate) 1000μg / ml, pH value of 6.5 wort special culture bottle, at 36 ℃ for 18 hours.

[0035] 3) secondary liquid seed culture; inoculating the primary liquid seed culture liquid into 1000 liters of sugar-containing (molasses) amount is 25% by weight, sel...

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Abstract

The present invention relates to one kind of selenium-rich and chromium-rich Saccharomyces cerevisiae, selenium-rich and chromium-rich Saccharomyces cerevisiae product and their production process. Of the selenium-rich and chromium-rich Saccharomyces cerevisiae product, each kilogram contains selenium in 100-4000 mg and chromium in 100-4000 mg. The selenium-rich and chromium-rich Saccharomyces cerevisiae may be used as selenium and chromium replenisher added into various food products or prepared into alone preparation. The selenium-irch and chromium-rich Saccharomyces cerevisiae product has lower toxicity than inorganic selenium and chromium product and high safety, and may be used in replenishing both selenium and chromium.

Description

technical field [0001] The invention relates to a selenium-chromium-enriched Saccharomyces cerevisiae, a selenium-chromium-enriched yeast product obtained from the selenium-chromium-enriched Saccharomyces cerevisiae and a production method thereof. Background technique [0002] Selenium and chromium are essential trace elements for the human body. They have important physiological functions for the human body, especially for maintaining blood sugar, blood lipids and blood pressure in the body and anti-cancer. Inorganic selenium and chromium are highly toxic to the human body, and yeast can absorb and utilize inorganic selenium and chromium in the medium during growth, and transform inorganic selenium and chromium into components in yeast cells. Transforming inorganic selenium and chromium into yeast selenium and yeast chromium can greatly reduce toxicity. There are many studies that separate selenium and chromium into production. However, pure selenium-enriched yeast and c...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12R1/865
Inventor 俞学锋李知洪余明华姚娟张彦匡金宝朱娅敏马灿许武桥夏长虹
Owner ANGEL NUTRITECH CO LTD
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