Method for breeding hybrid of rape and cruciferae vegetables by using wild leaf mustard sterile cytoplasm

Abstract

A process for preparing the hybrid seeds of rape and the vegetables in the mustard family by using the sterile cytoplasm of charlock includes such steps as separating the protoplast from leaf pulp, purifying it, pre-treating, fusing, culturing, discriminating the hybrid, reproduction, selectively culturing the male sterility recovery line of charlock cytoplasm, transferring the male sterility andrecovery characters from the charlock cytoplasm to rape or the vegetables in mustard family, and hybridizing between the charlock cytoplasm male sterility line of rape or the said vegetables and the male fetile rape or the said vegetables.

Description

technical field [0001] The invention belongs to the field of plant breeding and improved seed breeding, and more specifically relates to a method for preparing hybrids of rapeseed and cruciferous vegetables by utilizing the sterile cytoplasm of wild mustard. Production risk, increase yield of hybrids. Background technique [0002] The main crops of Cruciferae are rapeseed (including Brassica napus, Brassica napus, Brassica napus and Ethiopian mustard) used for harvesting seeds and extracting oil, as well as cabbage and various cabbage vegetables. In addition to being a high-quality edible oil, rapeseed oil is also an ideal raw material for biodiesel, so rapeseed has become an important field crop worldwide. There are many kinds of cabbage and cabbage in cruciferous vegetables. Cabbage includes Chinese cabbage, pakchoi, red cabbage, cabbage, etc., and cabbage includes head cabbage, broccoli, cabbage, kohlrabi, etc. Among them, many kinds of cabbage vegetables also have heal...

AI Technical Summary

Problems solved by technology

Radish male sterile material with relatively stable fertility obtained by transferring sterile cytoplasm of radish abroad (Bannerot H, Boulidard L, Cauderon Y and Tempe J. Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea. Proc. Eucarpia Meeting Cruciferae: 1974, 52-54), but in the process of utilization, it was difficult to find the restorer line, and the problems of linkage between the restorer gene and the bad quality traits made the breeding and utilization of the sterile line slow (Delourme R., Foisset N., Horvais R. , Barret P., Champagne G., Cheung W.Y., Landry B.S., Renard M. Characterization of the radish introgression carrying the Rfo restorer gene for the Ogu-INRA cytoplasmic male sterility inrapeseed (Brassica napus L). 1998, Theor Appl Genet 97: 129- 134)

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