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Detection chip for tanshinol related gene, preparation and detection method thereof

A detection chip and detection method technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., to achieve strong specificity and strong scientific effects

Inactive Publication Date: 2007-09-26
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no detection chip for Danshensu-related genes on the market

Method used

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Embodiment Construction

[0068] The present invention is further described in detail by the following examples, which are illustrative, not restrictive, and the protection scope of the present invention cannot be limited by the following examples.

[0069] In the gene chip for detecting the content of danshensu, probes for detecting danshenin are arranged on a matrix, and the matrix used in the gene chip is a glass slide.

[0070] The probe sequence is:

[0071] Danshensu related gene probe

[0072] number sequence

[0073] No. 1: 5′CTTGGCAGGTCGAGAACTTGCTACACGGGCCCTTATGCTCGTTCGGGTCCATGTCACATG3′

[0074] No. 2: 5′TCCAGTCGAAGTTATGAACCAGAGTCGCCGTAGCCATGTGCAGGATCCGGGTCGCGAGCG3′

[0075] No. 3: 5′AGCGACGATCCACACCACAGAGGGTCTAAAAATGACAGTTACGCGAAGGCAACGAGCTCC3′

[0076] No. 4: 5′CCGCCCATACTAGCACCCCGTAGTTGTGTCCGTTCCACCCGCGATCAAAAACCTGGCTCG3′

[0077] No. 5: 5′CCGCCCATACTAGCACCACCGTAGTTGTGTCCGTTCCACCCGCGATCAAAAACCTGGCTCG3′

[0078] No. 6: 5′TACTTCTTCAACCACTTCGCCAGGGCCATCACCATTCTCTC...

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Abstract

The invention discloses a detecting chip, making and detecting method of relative gene of salvia miltiorrhizae, which is characterized by the following: setting astopic probe on the glass to make gene chip; crossing with cDNA of marked sample of salvia miltiorrhizae; scanning result; analyzing expressive quantity of relative gene of salvia miltiorrhizae; fitting for obtaining functional information to pave the base to study salvia miltiorrhizae.

Description

technical field [0001] The invention belongs to the technical field of biochips, in particular to a detection chip for danshensu-related genes, a preparation method and a detection method. Background technique [0002] Salvia miltiorrhiza is the root of Salvia miltiorrhiza Bge, a labiatae plant, also known as blood root, red ginseng, blood ginseng, red root, and is an important traditional Chinese medicine. Danshensu is the main water-soluble component of Danshen. Its English name is Danshensu, its alias is B-3,4-dihydroxyphenyl lactic acid, its chemical name is Propanoic acid, 3-(3,4-dihydroxyphenyl)-2-hydroxy, and its molecular formula is C 5 h 10 o 5 . The main functions of Danshensu are: 1. It has the effect of dilating arteries; 2. The effect of anti-thrombosis; 3. The effect on the central nervous system: Danshensu can inhibit the activity of cyclonucleotide phosphodiesterase and improve the activity of brain. 4. Prevention and treatment of liver fibrosis; 5. It ha...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 王庆浩张伯礼王瑞菊
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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