Medicinal composition for prevention or treatment of parasitic protozoan infection
A technology for protozoa and infectious diseases, which is used in drug combinations, anti-infective drugs, medical preparations containing active ingredients, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0063] 1-1. Cultivation of Drug-Susceptible Tropical Fever Malaria Protozoa
[0064] In this example, a protozoan of Plasmodium falciparum F CR-3 strain was used. The medium used in the experiment was filter-sterilized RPMI-1640 medium, the pH of which was adjusted to 7.4, and human serum was added to a final concentration of 10%. The cultivation of malaria protozoa in O 2 Concentration 5%, CO 2 Concentration 5%, N 2 The concentration is 90%, and the temperature is 36.5°C.
[0065] 1-2. Drug-sensitive Tropical Fever Malaria Protozoan Proliferation Inhibition Screening Test
[0066] The cultured malarial protozoa-infected erythrocytes were collected by centrifugation, washed with serum-containing medium, and then non-infected erythrocytes were added. The initial infection rate was 0.3%. The hematocrit value at this time was 3%. The compound of the present invention used in the test and the positive drug (chloroquine, quinine) were dissolved in dimethyl sulfoxide (DMSO) as...
Embodiment 2
[0086] 2-1. Cultivation of chloroquine-resistant tropical malaria protozoa
[0087] A protozoa of Plasmodium falciparum K1 strain was used in this example. The culture medium used in the experiment is a culture medium formed by adding human serum at a final concentration of 5% to the filter-sterilized RPMI-1640 medium. The cultivation of malaria protozoa in O 2 Concentration 3%, CO 2 Concentration 4%, N 2 The concentration was 93%, and the temperature was 37°C.
[0088] 2-2. Chloroquine-resistant Tropical Fever Malaria Protozoan Proliferation Inhibition Screening Test
[0089] The compound of the present invention used in the test-positive object drug (chloroquine) was dissolved in DMSO to prepare a test solution with a predetermined concentration. The cultured malarial protozoa-infected erythrocytes were collected by centrifugation and diluted with non-infected erythrocytes. The initial infection rate was 0.15%. The hematocrit value at this time was 2.5%. Add 200 μL of...
Embodiment 3
[0109] 3-1. Culture of African trypanosomiasis protozoa
[0110] In this example, the bloodstream-dwelling trypanomastigotes of the protozoa of Trypanosoma brucei rhodensiense (STIB900 strain) were used. The medium used in the experiment was filter-sterilized MEM medium supplemented with 25mM N-2-hydroxyethylpiperazine-2-ethanesulfonic acid (HEPES), 1g / L glucose, 1% MEM non- Essential amino acids, 0.2 mM 2-mercaptoethanol, 2 mM sodium pyruvate, 0.1 mM hypoxanthine, and 15% heat-treated horse serum. Protozoa were cultured in CO 2 Concentration of 5% in the air, at a temperature of 37 degrees.
[0111] 3-2. African trypanosomiasis protozoan proliferation inhibition screening test
[0112] The compound of the present invention used in the test and the positive drug (Melarsoprol (Melarsoprol)) were dissolved in dimethyl sulfoxide (DMSO) to prepare a test solution with a predetermined concentration. In the wells of the 96-well culture plate, the number of protozoa containing 8×...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com