Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Determination method for entrapment efficiency of liposome

A measurement method and liposome technology, applied in the direction of testing pharmaceutical preparations, special data processing applications, instruments, etc., can solve the problems of long time consumption, large error in results, and high requirements for instruments, and achieve short time consumption, good accuracy, and experimental results. low cost effect

Inactive Publication Date: 2007-08-15
SHANGHAI UNIV OF T C M
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has high requirements for instruments, takes a long time, and has the disadvantages of large error in the results mentioned above.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Determination method for entrapment efficiency of liposome
  • Determination method for entrapment efficiency of liposome
  • Determination method for entrapment efficiency of liposome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 (determination of the encapsulation efficiency of the entire sennecline liposome by the reverse dialysis method of measuring the volume of the external aqueous phase with an auxiliary substance).

[0029] 1.1 Preparation of entire sennecline liposome samples

[0030] The whole-edge sennecline (A base) liposomes were prepared by dry film dispersion method. Take 0.2g of base drug A, 2g of injection-grade soybean lecithin, 0.5g of cholesterol, and 0.1g of vitamin E in a 500ml eggplant-shaped bottle, add 50ml of chloroform to dissolve, evaporate the chloroform under reduced pressure at 60°C and 50r / min to form Lipid dry film, vacuum-dried at room temperature for 12 hours to remove residual chloroform, hydrated the dry film with 50ml CBS 4.0, filled with nitrogen, shaken on the shaker to dissolve the film on the bottle wall, and then stood it in the refrigerator at 4°C for 12 hours, and finally used High-shear disperser disperses to make the whole system unifor...

Embodiment 2

[0065] Embodiment 2 (determination of the encapsulation efficiency of liposome by the centrifugation ultrafiltration method of measuring external aqueous phase volume with auxiliary substance)

[0066] 2.1 Effect of ultrafiltration on the concentration of free drug solution

[0067] Take three different concentrations (respectively 1.22, 0.50, 0.10mg / ml) of A alkali aqueous solution in three parts, 200 μL in each part, carry out ultrafiltration (Ultrafiltration unit adopts Microcon YM 100K, Millipore), and measure the filtration by HPLC respectively. The peak area corresponding to the base A in the solution and the solution before ultrafiltration. The results are shown in Table 5. It can be seen that the ultrafiltration membrane has a small amount of adsorption on the drug. When the drug concentration is large enough, it can be considered that the ultrafiltration has no effect on the concentration of the drug solution.

[0068] Table 5 The influence of ultrafiltration on the ...

Embodiment 3

[0080] Example 3 (determination of the encapsulation efficiency of the entire sennecline liposome by the reverse dialysis method of measuring the volume of the external aqueous phase with an auxiliary substance).

[0081] The test procedure and test conditions are the same as in Example 1, with phenol red replacing 5-Fu, and the test results are the same as in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

This invention discloses one test method with drug fat package rate, which comprises the following steps: adding the liquid not reacting with resin double molecule layer into resin mixture hanging liquid to achieve balance through dialysis water concentration to get outer phase for fat test; this invention adds double molecule layer without reaction into sample to be tested; filtering to get property liquid to test its medicine and liquid concentration to compute discrete bus to pack rate.

Description

technical field [0001] The invention relates to a method for measuring the drug encapsulation efficiency of a drug-containing liposome. Background technique [0002] Liposomes are closed vesicles with a bilayer structure made of phospholipids and (or without) additives as the skeleton membrane material. There are two long hydrophobic hydrocarbon chains and a hydrophilic group in the common phospholipid molecular structure. When an appropriate amount of phospholipid is added to water or buffer solution, the phospholipid molecules are aligned, and the hydrophilic groups face the water phase on both sides. , The hydrophobic hydrocarbon chains are relatively associated with each other and form a bilayer to form a liposome. The phospholipids used to prepare liposomes include natural phospholipids, such as soybean phospholipids, egg yolk phospholipids, etc.; synthetic phospholipids, such as dipalmitoylphosphatidylcholine, distearoylphosphatidylcholine, etc. A commonly used addit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15G06F19/00
Inventor 郑杭生徐莲英陈建明
Owner SHANGHAI UNIV OF T C M
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com